FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993081
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA32710.32937897311498254No Hit
GTATATTACTGTGCGAGGGAAAATGGATATAGTTATGGCTCTCCCTACCT29730.29937135037323237No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25420.25597106379036555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19060.19192794948246922No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18400.1852819659222158No Hit
CCTGATATATCGTGCATCCAGTTTGCAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTT17810.1793408594062317No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17720.1784345889207426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17670.17793110531769313No Hit
ATATATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTACACAACTGGTAACGCTCTGACTAGC17420.1754136873024456No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16930.17047954799256052No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14160.14258655638361825No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13990.14087471213324995No Hit
GTCTACAGCCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTGCATT13650.13745102363251335No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12480.12566950732115506No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12410.12496463027688579No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12090.12174233521736898No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11790.11872143359907199No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCCCTATGCCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG11550.11630471230443437No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11480.11559983526016508No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11250.11328381068613738No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT11200.1127803270830879No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11190.11267963036247798No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11190.11267963036247798No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10840.1091552451411315No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10830.10905454842052159No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10820.10895385169991169No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10710.10784618777320279No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10680.10754409761137308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10550.10623504024344439No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCTAGTCAGA10500.1057315566403949No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10410.1048252861549058No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10370.10442249927246619No Hit
TTCTTGGAGGGGTAATGCAACTCTGTTGACAGTAGTAAGTTGCAAAATCT10210.1028113517427078No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10140.1021064746984385No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10090.10160299109538899No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCTAGTCAGAGCGTTACCAGTTGTGTAAATTGGTATCAGCAGAAA10070.1014015976541692No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10040.1010995074923395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)