FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870448
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23810.2735373049280371No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19140.21988677095013143No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAAAGTTCAGCCTCAGGGAAA17460.2005863647225337No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17270.1984035806848887No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATT15600.17921805782769332No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15410.17703527379004833No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14880.17094645515872287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14420.16566182012021397No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG13760.15807951767365772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13530.15543720015440327No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13250.15222046578313694No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTGTT12850.14762513096704227No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12810.1471655974854328No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12750.14647629726301858No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11830.13590702718600078No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTTCCAACAGAAACCTGGCCGGGCTCCCAGGCT10950.12579729059059241No Hit
GTATATTACTGTGCGAGAGGGTCGGCTGCCACTTTTCACTACTGGGGCCA10920.12545264047938534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10800.12407404003455691No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10800.12407404003455691No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10730.12326985644174034No Hit
GTTTATTACTGTCAGCACCGTAGCATCTGGCAACTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA10690.12281032296013088No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10630.12212102273771668No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10580.12154660588570483No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10490.12051265555208353No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG10310.11844475488484091No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9960.11442383692075804No Hit
GACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCACCGTAGCATCTGG9870.11338988658713674No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC9850.11316011984633201No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9460.10867966840063968No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTT9400.10799036817822547No Hit
CCGTAGCATCTGGCAACTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT9380.10776060143742074No Hit
ATGCTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTC9120.10477363380695917No Hit
GTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9110.1046587504365568No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9090.10442898369575207No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8840.10155689943569289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT5550.056.04321770
CGAGACG2550.049.26433623
CGTATGC850.048.77369741
GACGCTC2750.046.94848326
GCGTATG306.312707E-445.99680740
CGCGTAT306.372877E-445.9083539
TCGCGTA306.439035E-445.8122438
ACGAGAC2850.045.2673322
CGCTCAT2950.044.9689828
GGTATCA9750.044.9113771
ATCTCGT1300.044.86340737
CCCACGA3050.043.40712419
CACGAGA3050.041.1858521
TGAATCG1000.041.03296334
CATGAAT3250.040.96421432
GAGACGC3250.040.7429424
CTTATAC3600.039.6020851
TATACAC4050.039.392553
CGAGCCC3500.038.7894215
AGACGCT3350.038.5220825
Produced by FastQC (version 0.11.5)