FastQCFastQC Report
Wed 8 May 2024
EGAF00004692446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870448
Total Bases64.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27570.31673345219932725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26270.30179861404701946No Hit
GTAATATACGGCCGTGTCCGCAGCGGTCACAAAGTTCAGCCTCAGGGAAA22660.2603257173317648No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22270.2558452658860725No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19300.2217249048765693No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATT17980.20656029998345682No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17380.19966729775931477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17020.1955314964248295No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC16650.19128081171994193No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15900.18266455893976435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15400.17692039041964597No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14960.1718655221219418No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14320.16451298641619028No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14270.16393856956417846No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTACTTAGCCTGGTTCCAAC14200.16313438597136187No Hit
GTATATTACTGTGCGAGAGGGTCGGCTGCCACTTTTCACTACTGGGGCCA13980.16060695182250978No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13880.1594581181184861No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13850.15911346800727902No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13150.15107163207911328No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13020.1495781482638825No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA12890.14808466444865173No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCAGTGGCCCTGTT12710.1460167637814091No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12250.1407321287429002No Hit
GTTTATTACTGTCAGCACCGTAGCATCTGGCAACTCACTTTCGGCCCTGG12220.1403874786316931No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12120.1392386449276694No Hit
GACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATTTTGCAGTTTA11570.1329200595555392No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11300.1298182085546753No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG11270.1294735584434682No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11250.12924379170266345No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10930.1255675238497877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10810.12418892340495928No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC10760.12361450655294745No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC10200.11718103781041486No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC10140.11649173758800067No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10130.11637685421759829No Hit
GTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGA9940.11419407017995331No Hit
TCTTTGATGCATCCAACAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGC9830.11293035310552728No Hit
CCGTAGCATCTGGCAACTCACTTTCGGCCCTGGGACCAAAGTGGATATCA9680.11120710254949176No Hit
CTCTTACACTGGGAGCACCAACTACAACCCCTCCCTCAAGAATCGAGTCA9570.10994338547506571No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG9400.10799036817822547No Hit
GTCTCTGGTGCCTCCATCAGTGATTACTACTGGAGCTGGATCCGGCAGCC9380.10776060143742074No Hit
ATGCTACGGTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCT9190.10557781739977574No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC9060.10408433358454496No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGACGCCA8780.10086759921327869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)