FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838934
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26700.3182610312611004No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25070.2988316124987186No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24790.295494043631561No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23870.28452774592518604No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTA22830.2721310615614577No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19730.23517940624649852No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT19000.22647788741426622No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18220.21718037414146998No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17760.21169722528828253No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTACAATAACTGGCTTTACACCTTTGGCCAGGGGACCAAG17620.21002844085470368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16850.2008501264700203No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16800.20025413202945644No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15900.18952623209930697No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15820.1885726409944048No Hit
GTCCCAGACCGACTGCCACTGAACCTGGCTGGGACACCAGAGGCCCTGGT15540.18523507212724719No Hit
CATCTATGGTGTATCCACCAGGGCCTCTGGTGTCCCAGCCAGGTTCAGTGGCAGTCGGTCTGGGACAGACTTCA15450.18416228213423225No Hit
GTACAATAACTGGCTTTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15140.18046711660273634No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14660.1747455699733233No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14420.17188479665861678No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTGCCGGCAACGTAGCCTGGTTCCAGCAGAAACCTGGCCAGGGTCCCAGGCT14110.16818963112712085No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14020.1671168411341059No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14010.16699764224599312No Hit
GTTTATTACTGTCAGCAGTACAATAACTGGCTTTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13200.15734253230885864No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC12900.15376656566547547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12750.1519785823437839No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12430.14816421792417522No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12250.14601863793814532No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12060.14375385906400265No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC11930.14220427351853662No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11810.14077388686118336No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11770.14029709130873227No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGCCGG11570.13791311354647684No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG11420.13612513022478526No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA11400.13588673244855973No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11390.13576753356044696No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11240.13397955023875538No Hit
CCATAGATGAGGAGCCTGGGACCCTGGCCAGGTTTCTGCTGGAACCAGGC11210.13362195357441706No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11130.13266836246951488No Hit
GTCCCAGGCTCCTCATCTATGGTGTATCCACCAGGGCCTCTGGTGTCCCA10870.1295691913785828No Hit
GTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCGACTGCCA10700.1275428102806657No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG10370.12360924697294425No Hit
GTTATTGTACTGCTGACAGTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT10280.12253645697992929No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG10230.12194046253936543No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGCCGGCAACGTAGCCTGGTT10010.11931808700088445No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9770.11645731368617794No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9520.11347734148335865No Hit
GGCCAAAGGTGTAAAGCCAGTTATTGTACTGCTGACAGTAATAAACTGCA9310.11097416483299043No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC9290.1107357670567649No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9290.1107357670567649No Hit
TCTATGGTGTATCCACCAGGGCCTCTGGTGTCCCAGCCAGGTTCAGTGGCAGTCGGTCTGGGACAGACTTCACT9120.10870938595884778No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.10668300486093066No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG8890.10596781153225403No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTACAATAACTGG8850.10549101597980294No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8780.10465662376301355No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8740.10417982821056244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8710.10382223154622412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8700.10370303265811137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT253.2076096E-668.58079539
CGAACGC200.00837331250.0857420
ATCGCGG750.050.0381937
GGTATCA11000.043.9937481
CGTATGC551.4741818E-743.8896541
AGCATCG3350.043.52400634
ACGAGAC6950.043.25238422
CTTATAC7000.042.910161
GCATCGG2000.042.47791735
CCCACGA7000.042.44096819
ATCTCCG7100.042.29570810
ATCTCGT1050.042.2400337
CACGAGA7000.041.9841321
GCCCACG7100.041.36319718
CGAGCCC6950.041.2906415
CGAGACT7250.041.0141723
AGACTCC7100.040.95908425
CGTGAAA809.858923E-1040.88303457
CATCGCG1500.040.8457536
TGCGTGA801.0550139E-940.6010355
Produced by FastQC (version 0.11.5)