FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838934
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60240.7180541019913367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28070.33459127893255014No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTA23420.2791637959601113No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21630.25782719498792517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20890.24900647726758007No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20410.243284930638167No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20020.2386361740017689No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18710.2230211196589958No Hit
GTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTACAATAACTGGCTTTACACCTTTGGCCAGGGGACCAAG18040.21503479415544013No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA17040.20311490534416296No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16940.20192291646303523No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16670.19870454648399039No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16370.19512857984060725No Hit
GTACAATAACTGGCTTTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT15550.18535427101535998No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15010.1789175310572703No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14740.17569916107822547No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14420.17188479665861678No Hit
GTCCCAGACCGACTGCCACTGAACCTGGCTGGGACACCAGAGGCCCTGGT14120.1683088300152336No Hit
CATCTATGGTGTATCCACCAGGGCCTCTGGTGTCCCAGCCAGGTTCAGTGGCAGTCGGTCTGGGACAGACTTCA13660.16282568116204613No Hit
CTCCTGCAGGGCCAGTGAGAGTGTTGCCGGCAACGTAGCCTGGTTCCAGCAGAAACCTGGCCAGGGTCCCAGGCT13390.15960731118300128No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG13120.15638894120395647No Hit
GTTTATTACTGTCAGCAGTACAATAACTGGCTTTACACCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12400.1478066212598369No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12270.14625703571437085No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11970.1426810690709877No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC11830.14101228463740892No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11770.14029709130873227No Hit
GTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACC11640.13874750576326622No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC11490.13695952244157467No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11330.13505234023177032No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11300.134694743567432No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG11240.13397955023875538No Hit
CCATAGATGAGGAGCCTGGGACCCTGGCCAGGTTTCTGCTGGAACCAGGC11150.1329067602457404No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11070.13195316914083824No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG11010.13123797581216162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10970.13076118025971054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10950.13052278248348498No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGCCGG10840.1292115947142445No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10730.127900406945004No Hit
GTCCCAGGCTCCTCATCTATGGTGTATCCACCAGGGCCTCTGGTGTCCCA10690.12742361139255293No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10650.12694681584010184No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10400.12396684363728254No Hit
GTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCGACTGCCA10340.12325165030860592No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA10250.12217886031559097No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10010.11931808700088445No Hit
GTTATTGTACTGCTGACAGTAATAAACTGCAGAGTCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT9950.11860289367220782No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9890.1178877003435312No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTGAGAGTGTTGCCGGCAACGTAGCCTGGTT9860.11753010367919288No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC9570.1140733359239225No Hit
GGCCAAAGGTGTAAAGCCAGTTATTGTACTGCTGACAGTAATAAACTGCA9330.11121256260921597No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC9220.1099013748399755No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGACTCTGCAGTTTATTACTGTCAGCAGTACAATAACTGG9070.10811339151828392No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG8790.10477582265112631No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8660.10322623710566027No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC8620.1027494415532092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT201.0059413E-466.7122817
GGTATCA11750.042.027081
GTATCAA30000.037.7036861
TACAACC451.2254687E-437.0402877
CGCGTTT400.002919281133.68367431
CCGCGTT400.002939161633.63706630
TATCAAC34000.033.2461362
AATGGTA3200.032.9243270
ATCAACG35300.031.5457133
TCAACGC35850.030.9632114
CAACGCA36200.030.849855
AACGCAG36500.030.5962896
CCCGCGT553.816311E-430.525929
TATAAGG553.9761607E-430.3129142
TAGCATC553.9817218E-430.3056875
TAGTACG450.00546794829.6304634
TGTTAGA9550.029.15661470
ACGCAGA38500.028.9202847
CGCAGAG38550.028.7098228
CGGGTAT704.8451147E-528.59097917
Produced by FastQC (version 0.11.5)