FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240618
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGAGCGAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGGTTTGCCC38030.3065407724214867No Hit
CTCCTGGACACCTGCCCGGTTTTCAGGCTCCCTCCTTGGGGGCAAACCTGCCCTGACCCTTTCGGGTGCGCAGC25670.20691300625978343No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG24190.1949834679167963No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23360.18829325384606704No Hit
CTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC23210.18708417901400753No Hit
AATATACACTGGGTCCGCCAGGCTTCCGGGAAAGGCCTGGAGTGGGTTGG22780.18361816449543694No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22270.17950731006643464No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21430.1727364910069014No Hit
GTCCAGGAGTGTCTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTG20760.1673359567570356No Hit
GAGTATTACTGCTCGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG20570.16580446196976023No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA20320.16378933724966105No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19430.156615493246108No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19260.15524520843644055No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18310.14758773450006368No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16470.13275641656013373No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16410.13227278662730993No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGGTTTGCCCCCAAGGAGGGAGCCTGAAAACCGGGCAGGTGTC16310.13146673673927026No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT16290.13130552676166232No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCACCACTATAGGAGAGCGAGCAGTAATACTCAG15510.1250183376349529No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14990.12082687821714662No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14830.11953719839628313No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA14830.11953719839628313No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14350.11566815893369273No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14070.11341121924718166No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTCGCTCTCCTATAGTGGTGCTTGGGTGTTCGGCGGAGGGACCAAG13830.11147669951588643No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13790.11115427956067056No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT13720.1105900446390428No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT13700.11042883466143487No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13220.10655979519884444No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13220.10655979519884444No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13180.10623737524362857No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12940.10430285551233337No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12700.10236833578103817No Hit
CCACTATAGGAGAGCGAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGGTTTG12640.10188470584821437No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12580.10140107591539056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3100.052.35136440
ACGAGAC3350.051.51626222
CGTATGC3300.050.34503641
GACTAGG3650.047.3412226
CGAGACT3800.046.31175223
ACTAGGC3850.045.78969627
ATCTCGT2850.045.772137
TCTCGTA2950.045.48652638
ATGCCGT3500.044.73200244
CACGAGA3900.044.2440121
GTATGCC3800.043.8055942
TGCCGTC3600.042.61133245
CTAGGCA4150.042.51394328
GGTATCA14550.042.09031
CTTATAC4350.042.002091
CGAGCCC4300.040.120215
GCCGTCT3750.040.0487146
GGATGGG9050.039.4386970
GATCTCG3350.038.8609736
AGGGGGG4250.038.7605670
Produced by FastQC (version 0.11.5)