FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439958
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGTGTCTGATCAGT62880.43667940314925846No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60610.42091505446686633No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51650.35869101737689574No Hit
GTAATATATAGCCGTGTCGTCAGTTCTCAGACTGTCCATTTGCAGATACA51300.3562603909280687No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41710.28966122623020946No Hit
GCCTTGGGCTGACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATAAGTGTACCTGCGTGTATATGAGCAG39650.27535525341711353No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC39190.27216071579865525No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37980.2637576929327105No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTTCTGCTGCTCATAT34280.23806249904511104No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC34050.23646523023588187No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29400.2041726217014663No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCAGTGGAAGCAGCAGTGATGTT28700.19931136880381234No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATAAGTGTAC27330.18979720241840387No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26830.18632487892007962No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26320.18278310895178887No Hit
CAGGTACACTTATGTCTTCGGAACTGGGACCAAGGTTACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCA26110.18132473308249267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25720.17861632075379974No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC25390.17632458724490574No Hit
GTCAGTCACCATCTCCTGCAGTGGAAGCAGCAGTGATGTTGGTGGCTATAATTATGTCTTCTGGTATCAAGTGCA24890.1728522637465815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24850.17257447786671554No Hit
ATATATTACTGTGCGAAAGACCATTACGAACATTGGAGTGGTTTTTTTGA23210.161185256792212No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23200.1611158103222455No Hit
GGATGAGGCTGACTATTTCTGCTGCTCATATACACGCAGGTACACTTATGTCTTCGGAACTGGGACCAAGGTTAC23070.1602130062126812No Hit
GTGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGTGGAAGCAG22850.1586851838734185No Hit
GTGTACCTGCGTGTATATGAGCAGCAGAAATAGTCAGCCTCATCCTCAGC21730.1509071792371722No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21430.14882378513817765No Hit
CCCCTGAGGGCCGCTTACCGACATCATAAATCAAGAGCTTGGGGGCTTTGCCTGGGTGCACTTGATACCAGAAGA21360.14833765984841224No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20660.14347640695075828No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20660.14347640695075828No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19670.13660120642407628No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19620.13625397407424383No Hit
CTCCTGCAGTGGAAGCAGCAGTGATGTTGGTGGCTATAATTATGTCTTCTGGTATCAAGTGCACCCAGGCAAAGC19520.13555950937457897No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGTTAC19480.13528172349471304No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG18920.13139272117658987No Hit
GACTCAGCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCACCATCT18870.13104548882675746No Hit
GCCCAGAGATGGTCAGGGAGGCCGTATTGCCAGACTTGGAGCCAGAAAAA18860.13097604235679094No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCAC18590.12910098766769584No Hit
GTTCTCAGACTGTCCATTTGCAGATACAGCGTGTTCTTGGAATTGTCTCT18390.12771205826836615No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18340.12736482591853374No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18180.12625368239906998No Hit
GACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATAAGTGTACCTGCGTGTATATGAGCAGCAGAAATAGT17790.12354527007037705No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17770.12340637713044407No Hit
GCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGTGGAAGCAGCAGTGATGTTGGTGG17590.12215634067104734No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17430.12104519715158359No Hit
ATATATTACTGTGCGAAAGACCATTACGAACATTGGAGTGGTTTTTTTGACGACTGGGGCCAGGGAACCCTGGT17430.12104519715158359No Hit
ACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATAAGTGTACCTGC17370.12062851833178467No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACGAGTGTGGCC17280.12000350010208632No Hit
CCTCAGGGGTCCCTGATCGTTTTTCTGGCTCCAAGTCTGGCAATACGGCCTCCCTGACCATCTCTGGGCTCCAGG17260.11986460716215334No Hit
GGCCACACTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17220.1195868212822874No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGGTTTTC16780.11653117660376205No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16730.11618394425392962No Hit
GCAGAAATAGTCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGG16670.11576726543413073No Hit
GCCCTCAGGGGTCCCTGATCGTTTTTCTGGCTCCAAGTCTGGCAATACGG16610.11535058661433181No Hit
CTATTTCTGCTGCTCATATACACGCAGGTACACTTATGTCTTCGGAACTG16420.11403110368496858No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACGAGTGTGGCCTTGTTGGCTTGGAG16210.1125727278156724No Hit
CTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16130.11201715605594052No Hit
GGGCACAGGATCGTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCAC16100.11180881664604107No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16040.11139213782624215No Hit
AGGTTACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG15880.1102809943067784No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTATTGCC15780.10958652960711354No Hit
CATATACACGCAGGTACACTTATGTCTTCGGAACTGGGACCAAGGTTACCGTCCTGGGTCAGCCCAAGGCCAACC15740.1093087437272476No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGT15370.10673922433848763No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15180.10541974140912444No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15170.10535029493915794No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15090.10479472317942606No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15050.10451693729956013No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14980.10403081200979472No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14810.10285022202036448No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14510.10076682792136993No Hit
GGTACACTTATGTCTTCGGAACTGGGACCAAGGTTACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACT14490.10062793498143696No Hit
GGGTAGAAGTCACTGATCAGACACACGAGTGTGGCCTTGTTGGCTTGGAG14470.10048904204150398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT300.002177759570.376170
CTACGCT2750.049.5338628
TCTGGAG16400.048.4908570
ACTACGC2750.048.26864627
ACGAGAC2900.048.0607922
CGAGACT2950.047.2494823
TACGCTG3050.044.6834429
CTTATAC3200.044.6282581
GACTACG3050.044.6120226
AGACTAC3050.044.60736525
CACGAGA3050.043.46650721
CATCGCG950.043.27669536
GCATCGC1050.042.3824335
GAGACTA3350.041.61353324
ATCGCGG504.0056657E-641.1517537
ACGCTGC3150.040.05211330
ATCTCCG3600.039.6571710
CGTATGC350.001348665939.42416441
TCTCCGA3650.039.1139211
TACACAT4000.039.0974275
Produced by FastQC (version 0.11.5)