FastQCFastQC Report
Wed 8 May 2024
EGAF00004692452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439958
Total Bases106.3 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGTGTCTGATCAGT60140.41765107037844157No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC59000.4097341728022623No Hit
GTAATATATAGCCGTGTCGTCAGTTCTCAGACTGTCCATTTGCAGATACA57850.4017478287561165No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56190.39021971474168No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA53840.37389979429955594No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC49440.34334334751430245No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT48460.336537593457587No Hit
ATATATTACTGTGCGAAAGACCATTACGAACATTGGAGTGGTTTTTTTGA46920.32584283708274825No Hit
GCCTTGGGCTGACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATA40360.280285952784734No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCAGTGGAAGCAGCAGTGATGTT40010.27785532633590704No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTTCTGCTGCTCATAT35840.24889614835988272No Hit
GGGCACAGGATCGTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT34430.2391041960946083No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33220.23070117322866363No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA33000.22917335088940097No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC32630.226603831500641No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31510.21882582686439467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30490.21174228692781316No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC30400.21111726869811479No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTAACCTTGGTC30340.21070058987831589No Hit
CAGGTACACTTATGTCTTCGGAACTGGGACCAAGGTTACCGTCCTGGGTC29990.20826996342948892No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG29350.20382538935163386No Hit
GTGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGTGGAAGCAG29030.20160310231270634No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28820.20014472644341016No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTCGTGT28290.19646406353518645No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG26640.1850053959907164No Hit
GGATGAGGCTGACTATTTCTGCTGCTCATATACACGCAGGTACACTTATG26270.18243587660195645No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCGT25280.17556067607527442No Hit
GTCAGTCACCATCTCCTGCAGTGGAAGCAGCAGTGATGTTGGTGGCTATA25270.17549122960530794No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24270.16854658260865943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT24220.16819935025882699No Hit
GGGCTGGAGTGGGTGGCAATTATCTGGTATGACGGAACTCAGAAATACTA23630.1641020085308044No Hit
CTCCTGCAGTGGAAGCAGCAGTGATGTTGGTGGCTATAATTATGTCTTCT23380.16236584678164223No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT22550.15660178977442396No Hit
GTCTGAGAACTGACGACACGGCTATATATTACTGTGCGAAAGACCATTAC22150.15382393097576458No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT22050.15312946627609972No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA21990.1527127874563008No Hit
GTGTACCTGCGTGTATATGAGCAGCAGAAATAGTCAGCCTCATCCTCAGC21630.15021271453750734No Hit
GTTCTCAGACTGTCCATTTGCAGATACAGCGTGTTCTTGGAATTGTCTCT21570.14979603571770844No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAGTTAC21190.147157069858982No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACGAGTGTGGCC20990.14576814045965228No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20870.14493478282005448No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG20780.1443097645903561No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCAC20720.14389308577055718No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20700.14375419283062424No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACTATGGCATGCACTGGG20670.1435458534207248No Hit
GGTATCAAGTGCACCCAGGCAAAGCCCCCAAGCTCTTGATTTATGATGTC20500.14236526343129455No Hit
GCCCTCAGGGGTCCCTGATCGTTTTTCTGGCTCCAAGTCTGGCAATACGG20250.14062910168213238No Hit
ACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATAAGTGTACCTGC19810.13757345700360704No Hit
GGCCACACTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG19760.13722622465377463No Hit
GCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCACCATCTCCTGCA19570.13590674172441142No Hit
GCACAGGATCGTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCC19550.13576784878447842No Hit
GACTCAGCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTCAGTCACCATCT19440.1350039376148471No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG19250.13368445468548387No Hit
CCTCAGGGGTCCCTGATCGTTTTTCTGGCTCCAAGTCTGGCAATACGGCC19000.13194829293632176No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18900.1312538282366569No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG18560.1288926482577964No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18540.12875375531786346No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGG18510.12854541590796398No Hit
CTATTTCTGCTGCTCATATACACGCAGGTACACTTATGTCTTCGGAACTG18440.1280592906181986No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18240.12667036121886888No Hit
TCCTCACTCAGGGCACAGGATCGTGGGCTCAGTCTGCCCTGACTCAGCCT18180.12625368239906998No Hit
GCACTGAACACAGAGGGCTCACCATGGAGTTTGGGCTGAACTGGGTTTTC17790.12354527007037705No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17600.12222578714101383No Hit
CATATACACGCAGGTACACTTATGTCTTCGGAACTGGGACCAAGGTTACC17470.12132298303144952No Hit
CTCGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17330.12035073245191873No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17300.12014239304201928No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGCGTCTCCTGGACAGTC17290.1200729465720528No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC17280.12000350010208632No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17250.11979516069218685No Hit
GGTACACTTATGTCTTCGGAACTGGGACCAAGGTTACCGTCCTGGGTCAG17240.11972571422222036No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC17230.1196562677522539No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA17090.11868401717272309No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17000.11805899894302473No Hit
CCCCTGAGGGCCGCTTACCGACATCATAAATCAAGAGCTTGGGGGCTTTG16890.1172950877733934No Hit
GACCATCTCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTTCTGCTGCT16790.11660062307372854No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16750.1163228371938626No Hit
GACCCAGGACGGTAACCTTGGTCCCAGTTCCGAAGACATAAGTGTACCTG16750.1163228371938626No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16740.11625339072389611No Hit
AGGTTACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC16670.11576726543413073No Hit
GGGTAGAAGTCACTGATCAGACACACGAGTGTGGCCTTGTTGGCTTGGAG16650.11562837249419775No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16620.1154200330842983No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCGTGGGCTCAGTCTGCCCTG16580.11514224720443236No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA16550.11493390779453289No Hit
GCTCATATACACGCAGGTACACTTATGTCTTCGGAACTGGGACCAAGGTT16530.11479501485459993No Hit
GCAGTGATGTTGGTGGCTATAATTATGTCTTCTGGTATCAAGTGCACCCA16480.1144477825047675No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC16420.11403110368496858No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC16410.11396165721500211No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16330.11340608545527021No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16330.11340608545527021No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16110.11187826311600754No Hit
GCCCAGAGATGGTCAGGGAGGCCGTATTGCCAGACTTGGAGCCAGAAAAA15930.11062822665661083No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15920.11055878018664433No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG15710.10910040431734815No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG15680.10889206490744868No Hit
GACTATTTCTGCTGCTCATATACACGCAGGTACACTTATGTCTTCGGAAC15600.1083364931477168No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA15280.1061142061087893No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTATTGCC15190.10548918787909092No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG14950.10382247259989527No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14920.10361413318999582No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA14910.10354468672002934No Hit
GGCAAAGCCCCCAAGCTCTTGATTTATGATGTCGGTAAGCGGCCCTCAGG14760.10250298967053206No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG14750.10243354320056558No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14750.10243354320056558No Hit
CTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTC14650.10173907850090072No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC14560.10111406027120236No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACGAGTGTGGCCTT14460.1004195955715375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)