FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868132
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65170.34885115184580107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59010.3158770365263268No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA50330.26941351039434047No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA46190.2472523354880704No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40830.21856057280748897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38700.207158808906437No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36960.19784469191684528No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36880.19741645665295598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36270.19415116276580027No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35610.19061822183871374No Hit
GTTAAGATACTGCTGACAGTAATAAACTGCAAAATCATCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT35430.1896546924949629No Hit
GTATTATTGTGCGCGACGGAATCGGGGACTCCTGAGTTTCTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCA34910.1868711632796826No Hit
GTCTGAGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGATGATT34600.18521175163211165No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34540.1848905751841947No Hit
CTCCTGCAGGGCCGGTCAGAGCATTGCCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT33190.17766410510606317No Hit
CTCCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCACTCTCACCATCAGCAG32690.1749876347067552No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31390.16802881166855446No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31040.16615528238903887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30940.1656199883091773No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30680.16422822370153714No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCACT29810.15957116520674128No Hit
GTATCTTAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29620.15855410645500426No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29030.15539587138382083No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCGGTCAGAGCATTGCCAC28310.15154175400881736No Hit
GTCTCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGA27360.14645646025013223No Hit
CAATTGTACTGTCTCTGGTGGCTCCATCAGCAGTGATAATTACTACTGGG27090.1450111662345059No Hit
CGTCTATGGTGCTTACTCCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGAGACAGAGTTCA26870.1438335192588104No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26250.1405146959636685No Hit
GTTTATTACTGTCAGCAGTATCTTAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA26050.13944410780394534No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25980.1390694019480422No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGATGATTTTGCAGTTTATTACTGTCAGCAGTATCTTAACTGG25650.13730293148449896No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25630.13719587266852665No Hit
GTCTGTGTCTCCAGGGGAAGGAGCCACCCTCTCCTGCAGGGCCGGTCAGAGCATTGCCACCAACTTAGCCTGGTA25520.1366070491806789No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25090.13430528463727404No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC24230.12970175555046431No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24190.1294876379185197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24100.12900587324664425No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT24010.12852410857476881No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23970.12830999094282416No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23580.12622234403136395No Hit
CCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC23550.12606175580740547No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA23490.1257405793594885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23310.12477705001573765No Hit
GTAATAAACTGCAAAATCATCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCTCAGACCCACTGCCA23200.12418822652788988No Hit
GTCTCTGGTGGCTCCATCAGCAGTGATAATTACTACTGGGACTGGATCCG23100.1236529324480283No Hit
CTATTATAGTGGGAGCACCCACTACAACCCGTCTCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCGAAGGA22850.12231469724837431No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22810.12210057961642967No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22230.11899587395323244No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20940.11209058032301784No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC20840.11155528624315628No Hit
ATCTTAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA20560.1100564628195438No Hit
GGATACCAGTGGCCCTGGAGTAAGCACCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG19900.10652352189245728No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19710.10550646314072025No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT19510.10443587498099706No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19440.10406116912509394No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19340.10352587504523236No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG19250.10304411037335692No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19180.10266940451745381No Hit
GCCCTGGAGTAAGCACCATAGACGAGGAGCCTGGGAGCCTGGCCAGGTTT19040.10191999280564756No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18870.10100999286988285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT454.474714E-1061.18829339
TATACGG350.004256290459.42922670
ATCGCGT700.058.85327537
GCGGTAT200.007745466751.08564424
TACGTAT1451.0913936E-1147.8166270
GCGTATG605.678885E-945.94095640
TATCGCG1350.045.7327936
AGTATCG2550.044.31599434
TCGCGTA704.6020432E-1044.18766838
GGTATCA16650.043.763711
ACCGATC5400.042.29113827
CTTATAC5800.042.268721
ACGAGAC5850.041.9085722
GTATCGC1400.041.61134735
CGTATGC1350.040.88403341
CCGATCA5600.040.79385828
AGACCGA5900.040.414325
GACCGAT5900.039.84976226
TATACAC6450.039.5832523
TCTACGG19400.039.3131570
Produced by FastQC (version 0.11.5)