FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387796
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35260.25407192411564816No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC29230.21062173403007362No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27370.19721918783452325No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25040.18042997674009725No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22940.16529806974512104No Hit
ACATGGGATAGCAGCCTGAGTGCTGCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGG20110.1449060236518912No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20030.14432957005208258No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG19850.14303254945251317No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAGGGGTTCCTGACCGATTCTCTGG19740.14223992575277633No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19720.14209581235282417No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA19080.13748418355435524No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16640.11990234876019241No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16520.11903766836047949No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTGCGGTGTTCGG16430.1183891580606948No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15800.11384958596220195No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15660.11284079216253685No Hit
GTAGTAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAGATACA15470.11147171486299139No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG15450.11132760146303924No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15310.11031880766337417No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15270.11003058086346984No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC14770.106427745864666No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14760.10635568916468992No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14460.10419398816540759No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14180.10217640056607742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.10210434386610137No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGCACTCAGG13960.10059115316660373No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13950.10051909646662766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG452.058663E-645.3329472
GGTATCA15450.042.0350381
GTATCAA44700.037.349131
CTAACGC558.174957E-637.0905953
GGATGGG10300.035.77662370
TATCAAC47150.035.550072
ATCAACG49350.033.896373
TCAACGC49500.033.7936554
TCGTATG1550.033.3829440
CAACGCA51000.032.9330525
AACGCAG51000.032.799726
CGTATGC1650.031.41831641
GCCGTCT1450.031.22399946
ACGAGAC3200.030.81668322
ATCTCGT1800.030.52001837
ACGCAGA54700.030.5145387
CGCAGAG55250.030.0866328
TAGTCAC1850.029.7438638
AGAGTAC56100.029.57017911
CAGAGTA56900.029.21417210
Produced by FastQC (version 0.11.5)