FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538547
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51130.33232653926074407No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCGCCCGCCGAATATCAC48270.3137375718778822No Hit
GACCTAGGACGGTCAGTTTGGTCCGCCCGCCGAATATCACAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG44480.28910394027611763No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40710.2646003014532543No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39040.2537459044150097No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37890.24627131962819465No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32090.2085734137468664No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCGCC31920.20746847512620675No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30240.19654908169851165No Hit
CTTCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACGATCTCCAG28170.1830948290822445No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACTGTGATATTCGGCGGGCGGACCAAACTGACCGTCCTAGGTCAG27680.17991000599916676No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27370.1778951179261992No Hit
GTGATATTCGGCGGGCGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG26820.17432031650641808No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26320.17107049703388977No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGAA26080.1695105836870762No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25930.1685356378453177No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25690.16697572449850412No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATCGTGAGGTAGC24990.16242597723696447No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24890.1617760133424588No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24300.15794122636487545No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24260.15768124080707316No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23700.15404144299784148No Hit
GTAATATATGGCCGTGTCTTCGGCTCTCAGGTTGTTCATCTGTAGAAACA23650.15371646105058864No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23310.1515065838092694No Hit
GAATATCACAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCAT23140.15040164518860977No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22880.14871173906289506No Hit
ATATATTACTGCGCGAGGCCAGATTTAGGGGAGTGGGCAATATTAAATTGGGGCCAGGGAACCCTGGTCACCGT22750.1478667860000377No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22200.14429198458025658No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21720.1411721578866294No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21520.1398722300976181No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21500.13974223731871696No Hit
GCTCTGGGGCTGAGCGCTACCTCACGATCTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC20950.13616743589893582No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACTGTGATATTCGGCGGGCGGACCAAACTGACCGTCCTAGGTCAGCC20680.13441253338377054No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20660.1342825406048694No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC20460.13298261281585808No Hit
GGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGTTCAC20430.1327876236475064No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20280.1318126778057479No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19430.12628798470244978No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19370.12589800636574638No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAGCGACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG19330.12563802080794412No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19110.12420810024003166No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18860.12258319050376751No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18400.11959335658904148No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT18170.11809843963167847No Hit
CCTGCACTCTGAGCAGTGGGCACAGCGGCTACGCCATCGCATGGCATCAG18030.11718849017937055No Hit
GCACAGGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTGCCTCT17910.11640853350596374No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17490.11367868514903998No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATCGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC17470.11354869237013884No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACTGTGATATTCGGCGGGCGGACCAAA17320.11257374652838036No Hit
ACCTAGGACGGTCAGTTTGGTCCGCCCGCCGAATATCACAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGC17280.11231376097057809No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16790.10912893788750035No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT16710.10860896677189581No Hit
TCCTCAGACTGGAGGCTGGAGATCGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC16530.10743903176178564No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16520.10737403537233506No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16450.10691906064618112No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16450.10691906064618112No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16270.10574912563607092No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16200.10529415090991695No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15960.10373423756310338No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15940.10360424478420224No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT15670.10184934226903695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)