FastQCFastQC Report
Wed 8 May 2024
EGAF00004692458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538547
Total Bases113.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56040.364239766480972No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46630.3030781640079893No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCGCCCGCCGAATATCAC46410.3016482434400769No Hit
GACCTAGGACGGTCAGTTTGGTCCGCCCGCCGAATATCACAGTGCCAGTG42000.2729848356923773No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41760.2714249223455637No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC41510.2698000126092996No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC38450.24991111743742633No Hit
GGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTGCCTCTGCCTC36690.23847175289412673No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCC33690.21897283605895693No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG33280.21630798409148375No Hit
GTGATATTCGGCGGGCGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGC33200.21578801297587918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33120.21526804186027468No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA32540.21149825127214184No Hit
GTAATATATGGCCGTGTCTTCGGCTCTCAGGTTGTTCATCTGTAGAAACA32440.21084828737763617No Hit
CTTCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG32360.21032831626203166No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACTGTGATATTCGGCGGGCG31330.20363368814862334No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31180.20265874230686484No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA30760.19992889394994107No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30680.19940892283433656No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA28990.1884245330171909No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28860.18757957995433353No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA27670.17984500960971617No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT27390.17802511070510033No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27210.17685517569499012No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGCATTGACTCAATCGC26680.17341036705411014No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATCGTGAGGTAGC25700.16704072088795469No Hit
ATATATTACTGCGCGAGGCCAGATTTAGGGGAGTGGGCAATATTAAATTG25400.16509082920443768No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTG24950.1621659916791622No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACTGTGATATTCGGCGGGCGGA24710.16060607833234863No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24520.1593711469327879No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGAA24470.15904616498553506No Hit
GCTCTGGGGCTGAGCGCTACCTCACGATCTCCAGCCTCCAGTCTGAGGAT24160.1570312769125675No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA23460.1524815296510279No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT23340.15170157297762107No Hit
GCACAGGGTCTCTCTCCCAGCTTGCATTGACTCAATCGCCCTCTGCCTCT23170.15059663435696147No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT23070.1499466704624558No Hit
GAATATCACAGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCAT22780.1480617751683894No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22630.1470868293266309No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG22170.14409699541190485No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22100.1436420206857509No Hit
CTCCTGTGCAGCCTCTGAATTCACCTTTAGCGACTATGCCATGACCTGGG21790.14162713261278337No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA21130.137337370909046No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20540.1335025839314626No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACTG20480.1331126055947592No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20280.1318126778057479No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20190.1312277103006928No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19900.12934281500662637No Hit
CCTGCACTCTGAGCAGTGGGCACAGCGGCTACGCCATCGCATGGCATCAG19510.12680795581805432No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT19460.12648297387080149No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18970.12329815078772374No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18900.12284317606156978No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18730.12173823744091015No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17900.11634353711651318No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17880.11621354433761205No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17840.11595355877980978No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA17730.11523859849585356No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17720.11517360210640298No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17470.11354869237013884No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17290.11237875736002864No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17070.11094883679211619No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAGATCGTGAGGTA17050.11081884401321507No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17000.11049386206596223No Hit
TCCTCAGACTGGAGGCTGGAGATCGTGAGGTAGCGCTCAGCCCCAGAGCT16940.11010388372925885No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16840.10945391983475318No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16610.10795900287739015No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG16490.10717904620398337No Hit
ACCTAGGACGGTCAGTTTGGTCCGCCCGCCGAATATCACAGTGCCAGTGC16480.1071140498145328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16190.10522915452046638No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG16020.10412421589980678No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16000.10399422312090563No Hit
ACTTTACAGTGATGGCCGCCACTTCAAGGGGGACGGGATCCCTGATCGCT15970.10379923395255394No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT15870.10314927005804828No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC15870.10314927005804828No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACGATCTCCAGCCTCCAGTCTG15840.10295428088969658No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15660.1017843458795864No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA15570.10119937837453129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)