FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055699
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30250.28654000808942703No Hit
GATTTATGCTGTGTCCAATTTGCAATATGGGGTCCCATCAAGGTTCAGCGGCAGCGGATCTGGGACAGACTTTT29130.27593092349239695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29060.2752678557050826No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24030.2276216989880638No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21980.20820328521671422No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC20070.1901110070199934No Hit
GCATAAATCAGGGACTTAGGGGCTTTCCCTGGTCTCTGCTGAAACCAGGC18280.17315541645866864No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18030.17078731721826013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17990.17040842133979478No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17920.1697453535524804No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGAGGCCAGTGTCAGGGAGA17910.16965062958286406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16600.1572417895631236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16420.15553675811002945No Hit
GTGTCTGGTGGGTCGTTCAGAGGTTACTTTTGGAGCTGGATCCGTCACTC16370.15506313826194779No Hit
GTATTACTGCCAACAGTCTGATGATTACCCTGTCACTTTCGGCGGCGGAA15870.1503269397811308No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15700.14871663229765303No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15170.143696261907987No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15140.14341208999913801No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15050.14255957427259097No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15040.14246485030297462No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT13960.13223466158440994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13910.13176104173632824No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13600.12882459867822174No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13530.12816153089090734No Hit
GTGTATTATTGTGCGAGTGTCAGTGGCTGGAGGGATTTTTGGGGCCGGGG13300.12598287958973153No Hit
GACCAGGGAAAGCCCCTAAGTCCCTGATTTATGCTGTGTCCAATTTGCAA13120.12427784813663742No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12850.12172030095699626No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12830.12153085301776359No Hit
GAACTAACGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12760.12086778523044922No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12620.11954164965582047No Hit
CACATGCACTGTGTCTGGTGGGTCGTTCAGAGGTTACTTTTGGAGCTGGA12460.11802606614195901No Hit
GTATTATTGTGCGAGTGTCAGTGGCTGGAGGGATTTTTGGGGCCGGGGAACCCGGGTCACCGTCTCCTCAGCATC12290.11641575865848125No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12200.11556324293193419No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11920.11291097178267669No Hit
CTGTGGGAGACACAGTCACCATCTCTTGTCGGGCGAGTGAGGGCGTTAGA11880.11253207590421134No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT11680.11063759651188454No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11610.10997452872457017No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11470.10864839314994142No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11430.10826949727147604No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGAGGCCAGTGTCAGGGA11400.10798532536262703No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11240.1064697418487656No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC11180.10590139803106757No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC11160.1057119500918349No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11050.10466998642605514No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10980.10400691863874077No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTTGCAGGTGTGACAT10920.10343857482104275No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10590.10031268382370354No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACGTTAGTTC10590.10031268382370354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)