FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055699
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48150.45609591370267477No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31300.29648602489914266No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25870.24505090939747032No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24130.22856893868422723No Hit
GATTTATGCTGTGTCCAATTTGCAATATGGGGTCCCATCAAGGTTCAGCGGCAGCGGATCTGGGACAGACTTTT23620.22373801623379394No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21000.19892033619431296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18520.17542879172946077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18190.17230290073212154No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17540.1661458427070595No Hit
GTGTCTGGTGGGTCGTTCAGAGGTTACTTTTGGAGCTGGATCCGTCACTC17210.16301995170972028No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGAGGCCAGTGTCAGGGAGA17200.16292522774010396No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16630.1575259614719726No Hit
GCATAAATCAGGGACTTAGGGGCTTTCCCTGGTCTCTGCTGAAACCAGGC16220.1536422787177027No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC16150.1529792109303883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15760.14928497611535105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14720.13943368327525174No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14370.13611834433867986No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14270.13517110464251647No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14250.1349816567032838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14130.13384496906788773No Hit
GTATTACTGCCAACAGTCTGATGATTACCCTGTCACTTTCGGCGGCGGAA14070.13327662525018968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13910.13176104173632824No Hit
GTGCAGCCACAGTTCGTTTGACCTCCACGTTAGTTCCGCCGCCGAAAGTG13900.13166631776671192No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13490.12778263501244197No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13410.12702484325551128No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12920.12238336874431065No Hit
GTGTATTATTGTGCGAGTGTCAGTGGCTGGAGGGATTTTTGGGGCCGGGG12670.12001526950390215No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12670.12001526950390215No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCTCCACGTTAGTTC12590.11925747774697143No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12250.1160368627800159No Hit
GTATTATTGTGCGAGTGTCAGTGGCTGGAGGGATTTTTGGGGCCGGGGAACCCGGGTCACCGTCTCCTCAGCATC12080.11442655529653811No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11720.11101649239034989No Hit
CACATGCACTGTGTCTGGTGGGTCGTTCAGAGGTTACTTTTGGAGCTGGA11610.10997452872457017No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11550.10940618490687212No Hit
GAACTAACGTGGAGGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11410.10808004933224337No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11300.10703808566646365No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT11160.1057119500918349No Hit
GGGTAATCATCAGACTGTTGGCAGTAATACGTCGCAGAATCTTCAGGCTG11120.10533305421336953No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11010.1042910905475898No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT11000.10419636657797346No Hit
TCATCAGACTGTTGGCAGTAATACGTCGCAGAATCTTCAGGCTGCAGGCTGTTGATGGTGAGAGAAAAGTCTGTC10970.10391219466912444No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT10890.10315440291219372No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10700.10135464748948328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3400.040.455461
GGTATCA9600.038.440911
CGTAGTT400.001535023838.39801467
TGGTATC3450.037.900952
CCTAACG755.1702955E-835.7629472
GTATCAA24700.035.3141331
GTATTGA701.2114506E-633.5484281
AATGGTA5700.032.98066370
TCAGCAT8500.032.32403670
TGGTACG500.00458425830.71841267
TATCAAC28200.030.674342
GCCGTTA553.8509603E-430.47978615
ATCAACG28350.030.2697753
TGTACGT450.005088475530.07030731
TCAACGC28950.029.6452314
CAACGCA29100.029.4952165
AACGCAG29300.029.4083146
TGGCTTG9600.029.37340570
TTAGGAC803.4553577E-629.3312363
GTAGTTT1504.4761455E-528.92150770
Produced by FastQC (version 0.11.5)