FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885873
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61650.32690430373625373No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC55900.2964144457235455No Hit
GTGATGGAGACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAAGTTT47840.253675618665732No Hit
CCTTTGGCCAGGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCA45490.2412145462605382No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44460.23575288473826178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44120.23395000617751038No Hit
GTAATACACAGCCGTGTCTTCAGTTGTCAGGCTGTTCATTTGCAGAGTCA41920.22228432137264809No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTGTACAGTGATGGAGACACCTACTTGA40540.21496675544959815No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38570.2045206649652442No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35450.18797660287834864No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34130.1809771919954313No Hit
GGCCAAAGGTGATCGGAGGCCAGTGTGTTGCTTGCATGCAGTAATAAAACCCAACATCCTCAGCCTCCACCCTG33660.17848497751439255No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33400.1771063056738179No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30350.16093342446707704No Hit
GTCTGGGACCCCAGAGTCCCGCCTAGAAACTTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA28960.15356283270400498No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28500.15112364406298834No Hit
GTCCCAGACAGATTCAGCGGCGGTGGGTCAGGCACTGATTTCACACTGAA28330.1502222047826126No Hit
GTGTATTACTGTGCGAAATTGGCCTTAAGGACGGTGACTTATGAATGGGA28270.14990404974248No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28250.1497979980624358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27340.14497264662042458No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27050.14343489725978367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26630.1412078119788554No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26400.13998821765834707No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC26130.13855651997775037No Hit
CTCCATCACTGTACACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC26080.13829139077763986No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25970.13770810653739674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25860.13712482229715361No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAAGTTTCTAGGCGGGAC25310.13420840109593807No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGG25280.13404932357587176No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCCTGGCCAAAGGTG25120.13320091013551813No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24480.12980725637410367No Hit
GCTAATGCTCTGGGTCCCAGGCTCCAGTGGGGATGTTGTGATGACTCAGT23130.12264876797112001No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22950.12169430285072218No Hit
GTAATAAAACCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACCGCCG22720.12047470853021386No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21530.11416463356758381No Hit
GTCCAAGGGTGACAGCCAGGGAGAGAGGAGACTGAGTCATCACAACATCC20590.10918020460550631No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGCTCCAGTGGGGATGTTGTGATGA20150.10684706764453385No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCAGTTATTTTGACATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG19880.10541536996393713No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19540.1036124914031857No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19520.1035064397231415No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGCTCCAGTGGGGATGTTGTGATGACTCAGTCTCCTCTCTCC19450.10313525884298677No Hit
TTATTACTGCATGCAAGCAACACACTGGCCTCCGATCACCTTTGGCCAGGGGACACGACTGGAAATTAAACGAA19400.10287012964287627No Hit
GTGTGTTGCTTGCATGCAGTAATAAAACCCAACATCCTCAGCCTCCACCC19300.10233987124265527No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCC19280.10223381956261106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19090.10122632860219112No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19060.10106725108212483No Hit
GTTTTATTACTGCATGCAAGCAACACACTGGCCTCCGATCACCTTTGGCC18950.10048396684188173No Hit
CCTCTCTCCCTGGCTGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAG18910.10027186348179332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2100.047.67403441
TCGTATG2200.045.4152240
GGTATCA17150.043.1776121
TACCTCG350.001333408239.5161542
TACGTAT1402.415436E-838.9898370
GTATCAA45850.036.449541
CCGTCTT2800.036.10786447
GCCGTCT2800.036.06466746
GTATGCC3050.035.14346342
ATCTCCG3000.035.08786810
ATGCCGT2900.034.69653344
AGACTAG3100.033.98483725
CTAACGC400.002805377133.9605033
TATCAAC49600.033.7550962
ACGAGAC3300.032.93838522
ATCAACG51600.032.446763
TCAACGC52450.031.9209294
GTGGTAT4900.031.8880521
GGTACGT1357.2759576E-1131.8184168
CAACGCA52650.031.7988325
Produced by FastQC (version 0.11.5)