FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885873
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63030.33422186965930367No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48170.2554254713864613No Hit
GTGATGGAGACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAAGTTT47710.2529862827454447No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44540.2361770914584386No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43090.22848834465523396No Hit
CCTTTGGCCAGGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCA41410.21958000353152093No Hit
GTAATACACAGCCGTGTCTTCAGTTGTCAGGCTGTTCATTTGCAGAGTCA38010.20155121792400657No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTGTACAGTGATGGAGACACCTACTTGA36970.19603653056170803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35920.1904688173593874No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34440.18262099303611642No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33010.17503829791295594No Hit
GGCCAAAGGTGATCGGAGGCCAGTGTGTTGCTTGCATGCAGTAATAAAACCCAACATCCTCAGCCTCCACCCTG30860.16363774230820421No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29900.1585472616660825No Hit
GTCTGGGACCCCAGAGTCCCGCCTAGAAACTTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA29870.15838818414601621No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28830.15287349678371767No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGG28480.15101759238294413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28350.15032825646265682No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28000.14847235206188328No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27920.14804814534170646No Hit
GTGTATTACTGTGCGAAATTGGCCTTAAGGACGGTGACTTATGAATGGGA27870.14778301614159597No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC27020.14327581973971737No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25780.13670061557697682No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25740.1364885122168884No Hit
GTCCCAGACAGATTCAGCGGCGGTGGGTCAGGCACTGATTTCACACTGAA25660.1360643054967116No Hit
CTCCATCACTGTACACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCC25280.13404932357587176No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCCTGGCCAAAGGTG25200.13362511685569495No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25130.13325393597554022No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24710.13102685069461198No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24510.12996633389416998No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24500.12991330805414786No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24490.12986028221412577No Hit
GTAATAAAACCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACCGCCG23070.1223306129309874No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22500.11930814004972763No Hit
GCTAATGCTCTGGGTCCCAGGCTCCAGTGGGGATGTTGTGATGACTCAGT21470.11384647852745121No Hit
GTGTGTTGCTTGCATGCAGTAATAAAACCCAACATCCTCAGCCTCCACCC21310.11299806508709759No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21250.112679910046965No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAAGTTTCTAGGCGGGAC21120.11199057412667768No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCAGTTATTTTGACATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG20900.11082400564619145No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20350.10790758444497589No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20300.10764245524486538No Hit
GTTTTATTACTGCATGCAAGCAACACACTGGCCTCCGATCACCTTTGGCC19840.10520326660384872No Hit
GTCCAAGGGTGACAGCCAGGGAGAGAGGAGACTGAGTCATCACAACATCC19780.1048851115637161No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19760.10477905988367192No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19650.1041957756434288No Hit
TTATTACTGCATGCAAGCAACACACTGGCCTCCGATCACCTTTGGCCAGGGGACACGACTGGAAATTAAACGAA19280.10223381956261106No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGCTCCAGTGGGGATGTTGTGATGACTCAGTCTCCTCTCTCC19180.10170356116239006No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGTCAAAATAAC18870.1000597601217049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.040.1725731
GTATCAA47250.039.708481
TTATCGT350.001466131938.7606117
TATCAAC51700.035.8768922
ATCAACG53000.034.9264263
TCAACGC53000.034.7957234
CAACGCA53700.034.40715
AACGCAG54800.033.717346
GTGGTAT5500.032.694381
TGGTATC5600.031.4871482
CGCAGAG58750.031.4503888
AGAGTAC59000.031.37458611
ACGCAGA58850.031.3393387
CGTGTCT13900.031.21666313
CAGAGTA59700.031.12028910
CAGCCGT15150.030.210549
AGTTGTC14200.030.11083822
ACCGTGT14100.030.09232750
GCCGTGT14900.030.0348211
AGCCGTG15350.029.59605210
Produced by FastQC (version 0.11.5)