FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010776
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23490.2323957038948293No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAGTCCTGCTGCTTGTATATGAGCTG21890.2165662817478848No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21670.21438973620267993No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAAGTCCTGCTGCTTGTATATGAGCTGCAGTAATAAT20490.20271553736930836No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20020.19806564461364337No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19800.19588909906843852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18590.1839180985698117No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17070.1688801475302144No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGGACTTTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT16970.16789080864603037No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16710.1653185275471519No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16490.16314198200194702No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACATT15800.1563155437010772No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15060.14899443595811535No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14820.14662002263607368No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14700.14543281597505284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14060.13910104711627502No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13660.1351436915795389No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT13310.13168100548489478No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13180.13039486493545552No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12990.12851512105550586No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12820.126833244952393No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12360.12228228608514646No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTAGTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGC12270.12139188108938083No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12230.12099614553570723No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12220.12089721164728882No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11650.11525798000743984No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC11440.11318036835065336No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA11310.11189422780121412No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11080.10961874836759083No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11010.10892621114866202No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10720.10605712838452831No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10680.10566139283085471No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10410.10299017784355784No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10330.10219870673621059No Hit
GTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAA10200.10091256618677134No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG10200.10091256618677134No Hit
GACCTGCACCGTCTCTGGATTCTCACTCAGCACTAGTGGAGTGGGTGTGG10150.10041789674467934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA452.006329E-645.50102216
GGTATCA10000.043.011241
GCCGTTA504.1442418E-640.94686515
GTATCAA26400.036.2047421
GGATGGG7850.035.63125270
TTAGTCG400.00234240635.23109456
TATCAAC28800.032.5885932
ATCAACG29100.032.1289863
GGTACCG751.8208029E-631.8428366
TCAACGC29300.031.7884984
CGTTAAA652.4983563E-531.50538617
TACCGCG652.5051251E-531.4929168
CAACGCA29600.031.4678735
TCAGGTC5000.031.38483412
AATGGTA7400.031.18336770
GTCATAG5100.030.7892441
AACGCAG30450.030.6999916
ACGCAGA31500.029.568357
CGCAGAG31900.029.0906358
GTCAGGT5300.028.96753911
Produced by FastQC (version 0.11.5)