FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514090
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT70770.46740946707263104No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51560.3405345785257151No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51250.33848714409315167No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50490.33346762741977026No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG46930.3099551545812997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40610.26821391066581246No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37760.24939072314063235No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37520.2478056126121961No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAACTATGTACATTGGTATCAGCAGAAA34020.2246894174058345No Hit
GATTTTATCTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAGGTTTATGATAATGT33770.22303826060538012No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33650.22224570534116203No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33230.2194717619163986No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33050.21828292902007143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32060.21174434809027204No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30060.19853509368663685No Hit
GTACATTAGCAACTATGTACATTGGTATCAGCAGAAACCGGGGAAAGCCCCTAAACTCCTGATCTACGATGCATC29860.19721416824627333No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29750.19648765925407338No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGTACATTAGCAA29590.19543091890178257No Hit
ACATATTACTGTCAGGTTTATGATAATGTCTACACTTTTGGCCAGGGGAC29540.19510068754169171No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29380.19404394718940088No Hit
AGTCAGTACATTAGCAACTATGTACATTGGTATCAGCAGAAACCGGGGAA29330.19371371582931002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29120.19232674411692832No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAGATAAAATCTGTCCCAGATCCACCTCCA29010.19160023512472837No Hit
ATATTACTGTCAGGTTTATGATAATGTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG28790.1901472171403285No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28390.18750536625960149No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28320.18704304235547425No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28020.18506165419492898No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27790.1835425899385109No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC27280.18017423006558395No Hit
ACTATGTACATTGGTATCAGCAGAAACCGGGGAAAGCCCCTAAACTCCTGATCTACGATGCATCCAATTTGGAAA26210.17310727895963912No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAGATAAAATCTGTCCCAGATCCACCTCCA25720.1698710116307485No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25620.16921054891056675No Hit
GTTCAGTGGAGGTGGATCTGGGACAGATTTTATCTTCACCATCAGCAGCC25120.16590823530965795No Hit
ATCATAAACCTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGC25050.16544591140553072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24910.16452126359727626No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24410.16121894999636746No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23930.158048728939495No Hit
GTGTAGACATTATCATAAACCTGACAGTAATATGTTGCAATATCTTCAGG23640.1561333870509679No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA23350.1542180451624408No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT22810.1506515464734593No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG22730.1501231762973139No Hit
GGCCAAAAGTGTAGACATTATCATAAACCTGACAGTAATATGTTGCAATA22560.1490003896730049No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC22480.1484720194968595No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22040.14556598352805974No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21700.14332041027944178No Hit
CCACTGAACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGAT21620.14279204010329635No Hit
GATAAAATCTGTCCCAGATCCACCTCCACTGAACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGAT21580.14252785501522366No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20690.13664973680560602No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT20540.13565904272533336No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20250.13374370083680626No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20050.13242277539644273No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT19780.130639526051952No Hit
GTTTAGGGGCTTTCCCCGGTTTCTGCTGATACCAATGTACATAGTTGCTA19750.13044138723589746No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19490.1287241841634249No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGT19310.12753535126709772No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19280.1273372124510432No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19170.12661070345884326No Hit
TGTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG19150.12647861091480692No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAACTTCAGGGAGA19100.12614837955471603No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19000.12548791683453428No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18220.12033630761711656No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18220.12033630761711656No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18120.11967584489693478No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18050.11921352099280756No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17340.11452423567951707No Hit
ACCTTGATGGGACCCCTGTTTCCAAATTGGATGCATCGTAGATCAGGAGTTTAGGGGCTTTCCCCGGTTTCTG17280.11412795804740801No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17250.11392981923135348No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17200.11359958787126259No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17130.11313726396713537No Hit
GTTTATGATAATGTCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16620.10976890409420839No Hit
GTGGAGGTGGATCTGGGACAGATTTTATCTTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTA16620.10976890409420839No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16330.1078535622056813No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16160.1067307755813723No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG16140.10659868303733595No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC16010.10574008150109966No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT15980.10554194268504515No Hit
ATGTACATTGGTATCAGCAGAAACCGGGGAAAGCCCCTAAACTCCTGATC15750.10402287842862709No Hit
CCCCTAAACTCCTGATCTACGATGCATCCAATTTGGAAACAGGGGTCCCA15720.10382473961257258No Hit
CATCAAGGTTCAGTGGAGGTGGATCTGGGACAGATTTTATCTTCACCATCAGCAGCCTGCAGCCTGAAGATATTG15700.1036926470685362No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15600.10303218434835446No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15480.10223962908413635No Hit
GCCTGAAGATATTGCAACATATTACTGTCAGGTTTATGATAATGTCTACA15300.10105079618780918No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15220.10052242601166378No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15150.10006010210753655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)