FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117579
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24010.21483939837810126No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20670.18495336794982725No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC19260.17233681019417868No Hit
GTTTTATGCTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG18840.16857868660738973No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT18250.16329941775928145No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18150.1624046264290936No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16500.1476405694809942No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT16190.14486671635741188No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGAATTGTCAGCAACTATGTG16150.14450879982533674No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15960.14280869629797985No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14820.13260807513383843No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14350.12840255588195557No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTGGTGCTATCATAAGACTGACAGTAGTAGTCAG14260.1275972436847865No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG14180.1268814106206362No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13870.12410755749705389No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13740.12294432876780971No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13590.12160214177252794No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13170.117844018185739No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13010.11641235205743844No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12860.11507016506215668No Hit
GTCTTATGATAGCACCAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12460.1114909997414053No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12450.11140152060838651No Hit
GTAATATACGGCCGTGTCCCCGGCTCTCAGGCTATTCATTTGTAGATACA12390.1108646458102738No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12140.1086276674848042No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGGTGTCGAGGGAGCCA11850.10603277262725946No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11690.10460110649895892No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11400.10200621164141417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC800.050.9633523
AACGTCG352.1380378E-549.7977947
CCTAACG850.047.965512
CGAGACG4200.047.73612623
TCTCGTA3550.046.4753638
ACGAGAC4450.045.80975322
TCGTATG3900.045.0709540
CGTATGC3900.044.27161841
ATCTCGT3750.043.01581237
ATGCCGT4100.042.3102344
GCCGTCT3800.042.1351746
CACGAGA4650.041.64747221
TATACAC5150.040.9026223
TATGCCG4350.040.62049543
AGACGGA5000.040.1145125
GGTATCA19600.039.0043071
GGAGCTA5000.038.8433929
TGCCGTC4400.037.9107945
CTTATAC5400.037.752321
CGAGCCC5250.037.52819415
Produced by FastQC (version 0.11.5)