FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632148
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG47250.2894958055274399No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTCCATCTGCAGATACA40460.24789418606645966No Hit
GTATATTACTGTGCGAAAGGAAAGGAAGTCGAATCTAGACTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT40330.2470976896702995No Hit
GATCTATACTGCATCCAGTTTGCAAAGTGGGGTCCCCTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA38370.23508897477434645No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37350.2288395415121668No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT37240.2281655830231082No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36830.2256535559275262No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG36510.22369294941390117No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA36160.22154853603962385No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGGACTGAC32060.19642826508380365No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28270.17320733168805771No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28130.17234956633834678No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT25760.1578288243468117No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24420.14961878457100705No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23680.14508488200824926No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22890.14024463467773757No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22620.13859037293186646No Hit
GCCATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC22530.13803895234990946No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT22170.13583327002208131No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21510.1317895190877298No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGG21420.13123809850577275No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21220.13001271943475715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20910.12811338187468294No Hit
GTCCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATACTGCATC20550.12590769954685482No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20430.12517247210424545No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20150.12345694140482358No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT20070.12296678977641734No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20000.12253790710156187No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19880.12180267965895249No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGTCCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA19770.12112872116989391No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGC19480.11935192151692126No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19480.11935192151692126No Hit
GTACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCTGCTTTTAGTGGTGGTGTTATTAAAACATA19400.11886176988851502No Hit
GAGTTACAATACCCCTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT18540.11359263988314784No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG18250.1118158402301752No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCCTACGTTCGGCCAAGGGAC17960.11003904057720255No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17940.10991650267010099No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17760.10881366150618695No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17730.1086298546455346No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTTCTTGT17700.10844604778488226No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17620.10795589615647601No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17560.10758828243517132No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17520.10734320662096819No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT17370.10642417231770647No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGTCCATTAGCAG17350.10630163441060492No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC17280.10587275173574945No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT17200.10538260010734321No Hit
CCTGTGAGGTGGCTGCGTACTTGCCCCCTCTCAGGACTGATGGGAAGCCC17130.10495371743248774No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16870.10336072464016745No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16630.10189026975494868No Hit
GTAGTAAGTTGCAGAATCTTCAGGGTGCAGATTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA16630.10189026975494868No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC16510.10115504231233932No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16440.10072615963748385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA100.008438015136.8021970
TCGCGTA252.6005323E-455.05732338
CGTATGC1950.049.57278441
GTGGTAT3050.049.232661
TCGTATG1900.045.37273440
GCGTATG551.4758916E-743.8878140
CTTATAC3900.043.75281
CGCGTAT405.0475872E-543.06010439
ACGAGAC3550.041.34348322
ATCTCGT1700.040.4445937
CCCTAAG3850.039.94154728
ATGCCGT2300.039.1556844
TAAGACA4050.037.18240731
TATACAC4650.035.956473
ACCCTAA4100.035.8314527
CACGAGA4250.035.3349221
GTATCAA26900.035.015251
CGAGACC4200.034.9461623
CGAGCCC4600.034.86160315
TGCCGTC2500.034.68086245
Produced by FastQC (version 0.11.5)