FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1632148
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG50590.3099596360134007No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44530.2728306501616275No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTCCATCTGCAGATACA43480.26639741003879547No Hit
GTATATTACTGTGCGAAAGGAAAGGAAGTCGAATCTAGACTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT39200.24017429791906128No Hit
GATCTATACTGCATCCAGTTTGCAAAGTGGGGTCCCCTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA38500.2358854711705066No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37190.22785923825535428No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA37150.22761416244115118No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT36790.22540848011332304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32540.1993691748542412No Hit
GTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGGACTGAC32080.19655080299090522No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30820.18883091484350684No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28250.17308479378095612No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC27550.1687959670324015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25120.1539076113195617No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT25080.15366253550535858No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGG21880.13405647036910867No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21530.13191205699483136No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21410.13117682955222199No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21270.13031906420251105No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21100.12927749199214777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20920.1281746508282337No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC20790.12737815443207356No Hit
GCCATGTACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC20570.1260302374539564No Hit
GTCCATTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATACTGCATC20430.12517247210424545No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC20410.12504993419714389No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT20330.12455978256873763No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC20170.12357947931192514No Hit
GTACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCTGCTTTTAGTGGTGGTGTTATTAAAACATA19780.12118999012344468No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19710.12076110744858923No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT19260.11800400453880408No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGTCCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA18780.11506309476836658No Hit
GAGTTACAATACCCCTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT18670.11438913627930801No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18620.1140827915115541No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18470.11316375720829237No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC18410.11279614348698769No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18190.11144822650887051No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18070.11071299906626116No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGGTCAGTCCATTAGCAG17660.10820097197067913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17610.10789462720292523No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17470.1070368618532143No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC17290.10593402068930023No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT17180.10526006220024164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17140.10501498638603851No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16920.10366706940792134No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT16900.10354453150081977No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGC16870.10336072464016745No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT16730.10250295929045651No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC200.007600494751.3300931
GTGGTAT3450.046.4628261
CCGTTTA408.3058013E-443.50620369
TCGACTA350.001424834538.98629820
GTATCAA28450.038.8409271
CGTGTTG806.214577E-835.0249752
CGACTAT400.002743904234.1140621
TATCAAC32550.033.5109062
ATCAACG33100.032.85113
TCAACGC33250.032.8024024
CGTTTAA850.003347146632.75632570
CAACGCA34200.031.8931775
AACGCAG34750.031.487456
TTAGTCG450.004904581730.298525
CAACCGA1052.2286258E-829.7098844
CGCAGAG36950.029.5204358
TCGACTC17000.029.49772320
CTCGACT17400.029.40686819
TCTGGAG21150.029.2909770
AGAGTAC37400.029.07410211
Produced by FastQC (version 0.11.5)