FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678397
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTGGCTCACCGGGGACGTTCGGCCAAGGGA64200.38250783336719496No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43000.2561968354328565No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42540.25345612510031895No Hit
CAATAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGC40750.24279118706718375No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33190.19774820855852338No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG31620.188394045032254No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31150.18559375404031345No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28660.170758169848969No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27660.16480010390867No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTAGAGCCTGAAGATT26270.1565183922516544No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25470.1517519394994152No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24250.14448309905225046No Hit
GTCCCAGACCCACTGCCAATGAACCTGTCTGGGAAGCCATTGGTCCTCTT24230.14436393773344447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23390.13935916234359333No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23180.13810796849613052No Hit
GTATGGTGGCTCACCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23110.1376909038803096No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTACCAGCAACTTCTTAGCCTGGTACC22980.13691635530807073No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22470.13387774167851826No Hit
TCTATGATGCGTCCAAGAGGACCAATGGCTTCCCAGACAGGTTCATTGGCAGTGGGTCTGGGACAGACTTCGCT21680.12917086958568205No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21370.12732386914418936No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21320.1270259658471744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21280.12678764320956246No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTACCAG20520.12225951309493523No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20470.12196160979792028No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20240.12059125463165152No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19720.11749306034269603No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19350.11528857594478542No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19300.11499067264777046No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19190.11433528539433757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18930.11278618824985985No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18440.10986673593911334No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18110.10790057417881466No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCAACTTCAACAA17730.10563650912150105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17580.1047427992304562No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17260.10283621812956054No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17200.10247873417314259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC4900.043.1847231
ACGAGAC4750.042.3966722
TCGTATG2600.041.14205640
CGTATGC2500.040.07432641
CACGAGA5100.039.48709521
GCCGTCT2450.038.28103646
TATACAC5700.037.7157363
TTATACA5750.037.387772
ATGCCGT2800.037.13207644
CCCTAAG5250.036.480228
CGAGACC5800.035.31517423
GGTATCA23350.034.3491131
AACTGTG28150.033.84533370
TCCGAGC6100.033.55835713
GACCCTA5850.033.28846426
TGCCGTC3250.033.10214645
GTATCAA50550.032.340661
TACACAT6650.032.3277745
CGAGCCC6350.032.2381215
TAAGACA6000.031.9841731
Produced by FastQC (version 0.11.5)