FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678397
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTGGCTCACCGGGGACGTTCGGCCAAGGGA61140.36427615158988014No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45140.2689470965450963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44760.26668303148778266No Hit
CAATAATACACTGCAAAATCTTCAGGCTCTAGTCTGCTGATGGTGAGAGC38000.22640650573136153No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37200.22164005297912234No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32710.19488833690717988No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30370.18094646260688024No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28910.17224768633404372No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26800.15967616720001288No Hit
GTCTGGGACAGACTTCGCTCTCACCATCAGCAGACTAGAGCCTGAAGATT26540.15812707005553514No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25590.15246690741225108No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24870.14817709993523584No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24850.14805793861642982No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23950.14269567927016075No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23800.1418019693791159No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23220.1383462911337425No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTACCAGCAACTTCTTAGCCTGGTACC22940.13667803267045878No Hit
GTATGGTGGCTCACCGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT22780.13572474212001093No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22490.13399690299732422No Hit
GTCCCAGACCCACTGCCAATGAACCTGTCTGGGAAGCCATTGGTCCTCTT22090.13161367662120463No Hit
TCTATGATGCGTCCAAGAGGACCAATGGCTTCCCAGACAGGTTCATTGGCAGTGGGTCTGGGACAGACTTCGCT20360.12130622254448739No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTACCAG20130.11993586737821862No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19910.11862509287135284No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19350.11528857594478542No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19010.11326283352508376No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18930.11278618824985985No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18710.11147541374299406No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18530.11040296187374023No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18340.10927092934508342No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCAACTTCAACAA17840.10629189637493394No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17770.105874831759113No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17760.10581525109971002No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.10551734780269506No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17680.1053386058244861No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC17280.10295537944836651No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17060.10164460494150072No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16980.10116795966627681No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16900.1006913143910529No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16870.10051257241284392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT851.233564E-648.82826270
CCTAACG451.0746835E-437.878582
GGTATCA25050.033.907811
AATGGTA9300.033.47098570
TCAACGC48400.032.1697854
ATCAACG48350.032.1412053
AACTGTG25700.031.76053670
GTATCAA51000.031.3697341
CAACGCA49650.031.2912465
AACGCAG50100.031.2142036
ACGCAGA52550.029.6940927
CGCAGAG52800.029.5534958
TATCAAC55450.028.834172
AGAGTAC53600.028.79457911
CTAACGC605.826602E-428.399623
GCAGAGT55150.028.294199
GTACGTA955.998936E-527.71030669
GACCGGA1256.78243E-427.6693570
ATAGGTG10350.026.992984
CAGAGTA58950.026.2969210
Produced by FastQC (version 0.11.5)