FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440681
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT65790.4566590383297898No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA52910.3672568736590543No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGAGTGGTG51110.35476278232308195No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46380.32193108675688786No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCACCATCTCCTG44960.31207463692517634No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43790.3039534775567943No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43670.30312053813439616No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38120.2645970898484814No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37890.26300062262221824No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37340.2591829836028933No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT33240.23072422000428966No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32520.2257265834699007No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30570.21219131785593062No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAGAGC30510.21177484814473158No Hit
GATTATTACTGCAGTTCATATACAAGCAGCACCACTCCTTATGTCTTCGG29550.2051113327655463No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29320.20351486553928316No Hit
CTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC28360.1968513501600979No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27480.19074312772917806No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27200.18879960241024904No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26770.1858149028133223No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCAC26680.1851901982465237No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25280.17547257165187852No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC24970.17332081147734996No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC24680.17130787453988774No Hit
CATAAGGAGTGGTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCTCG23300.16172907118230892No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22580.15673143464791997No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22200.15409379314365915No Hit
GTTCATATACAAGCAGCACCACTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGG22150.15374673505099326No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22090.15333026533979416No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCACCATCT21980.1525667375359292No Hit
CCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC21980.1525667375359292No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACATCA21760.15103968192819922No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCGGAGAAG21530.1494432147019361No Hit
GGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAGAGCCCCCAAACTCATGATTTATGAAGTCAGTAAT21200.14715263129034117No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21180.1470138080532748No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGAGTGGTGCTGCTTGTATATGAA21160.14687498481620845No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCAC19830.13764323955129554No Hit
GTACCAACATCACCCAGGCAGAGCCCCCAAACTCATGATTTATGAAGTCA19460.13507500966556787No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG19210.1333397192022384No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19100.1325761913983734No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18750.1301467847497121No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA18650.1294526685643803No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18450.1280644361937167No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18380.12757855486398448No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18090.12556561792652224No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17680.12271974156666188No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17060.11841622121760473No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17030.11820798636200519No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17030.11820798636200519No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16670.11570916809481072No Hit
GACATAAGGAGTGGTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCT16570.11501505190947893No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCC16420.11397387763148123No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16270.11293270335348353No Hit
CCCAGGCAGAGCCCCCAAACTCATGATTTATGAAGTCAGTAATCGGCCCTCAGGAATTTCTAATCGCTTCTCCGG16230.1126550568793508No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16140.11203035231255219No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG15640.1085597713858932No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15610.10835153653029365No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15410.10696330415963005No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT15400.10689389254109688No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15250.10585271826309918No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15140.10508919045923422No Hit
CTCCTGGTCAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT15090.1047421323665683No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15040.1043950742739024No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14860.10314566514030518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.048.273891
TCGACGT3250.046.01809729
ACTCGAC3400.045.9409827
CTCGACG3400.044.9641428
GTGGTAT3550.043.9448171
AGACTCG3650.043.69275325
GACTCGA3600.043.36910626
TCGTATG1600.043.04548340
TGGTATC3950.041.2090072
CGTATGC1850.041.03238341
ATGCCGT1900.040.16396344
ATCTCCG4050.039.34461610
CGAGACT4150.039.2284823
CGACGTC3750.039.00579530
ACGAGAC4000.038.99831422
TATACAC4350.038.978833
GACGTCA3850.037.1388531
TCTCCGA4300.037.05714411
CTAACGC558.322004E-636.9944533
ATGTCGT754.251706E-836.56842837
Produced by FastQC (version 0.11.5)