FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440681
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT57050.3959932837317907No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC53070.36836745955558514No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA50760.35233337567442063No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC48710.33810399387511875No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCACCATCTCCTG45500.31582286432596807No Hit
GACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGAGTGGTG44780.3108252277915791No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41920.2909735048910897No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG41790.29007115385015836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37430.25980768816969196No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34050.2363465611054772No Hit
GTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33570.23301480341588457No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31840.22100659340964446No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT31640.21961836103898086No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29800.2068466232288758No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29400.2040701584875486No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAGAGC29140.20226545640568594No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCAC27780.19282547628517346No Hit
GATTATTACTGCAGTTCATATACAAGCAGCACCACTCCTTATGTCTTCGG27660.19199253686277531No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27580.19143724391450986No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27420.190326658017979No Hit
CTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCC26990.18734195842105228No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26740.1856066679577228No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTTAGGACGGTGACCTTGGTC26730.1855372563391896No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG25220.17505610194067944No Hit
GCCTTGGGCTGACTTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGGAGTGGTGCTGCTTGTATATGAA23610.16388083135683748No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23060.1600631923375126No Hit
CATAAGGAGTGGTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCTCG22610.15693966950351954No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21850.15166438649499783No Hit
GTTCATATACAAGCAGCACCACTCCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGG21620.15006791926873472No Hit
GGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAGAGCCCCCAAACTCATGATTTATGAAGTCAGTAAT21400.14854086366100475No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACATCA21130.1466667499606089No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21000.14576439891967757No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20830.1445844014046135No Hit
TCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20700.14368205036368217No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20610.14305734579688353No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCACCATCT20160.13993382296289048No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCGGAGAAG19440.13493618642850153No Hit
CCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC18870.13097972417211026No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18860.13091031255357707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT18780.13035501960531165No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA18690.12973031503851304No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18620.12924443370878078No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGTCAGTCGATCAC18390.12764796648251767No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18100.12563502954505543No Hit
GTACCAACATCACCCAGGCAGAGCCCCCAAACTCATGATTTATGAAGTCA17250.11973504196973515No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17200.11938798387706925No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17200.11938798387706925No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17020.118138574743472No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16790.11654210751720889No Hit
GACATAAGGAGTGGTGCTGCTTGTATATGAACTGCAGTAATAATCAGCCT16500.11452917057974665No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16430.11404328925001442No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16220.11258564526081763No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16180.1123079987866849No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16040.11133623612722039No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15930.1105727083233554No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCC15780.10953153404535772No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG15680.10883741786002592No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15650.1086291830044264No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15610.10835153653029365No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15350.10654683444843098No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15320.10633859959283144No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15290.10613036473723192No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15250.10585271826309918No Hit
GTGCAGGAGATGGTGATCGACTGACCAGGAGACCCAGACACGGAGGCAGG14690.10196566762524112No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG14560.10106331658430978No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA150.002525385767.7998819
AATCGAC252.2915595E-456.48452853
AATGGTA8100.048.0847570
GGTATCA15450.047.621781
GTGGTAT4300.046.521951
TGGTATC4600.040.5156482
GTATCAA43350.037.3081441
TATCAAC47400.034.0287932
TACGTAT1501.3617064E-633.0473470
ATCAACG48750.033.0146563
TCAACGC49000.032.7747884
CAACGCA50650.031.839785
AACGCAG51350.031.3397626
GAGTTTA2950.029.8831041
CGCAGAG54350.029.609888
ACGCAGA54600.029.47437
TATACTG704.3589265E-529.0399465
CCGAAAC953.8157195E-628.9606468
AGAGTAC54750.028.96038211
AGTAATA33650.028.83136470
Produced by FastQC (version 0.11.5)