FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836976
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA67740.36875822003118164No Hit
GTGTATTACTGTGCGTCACCCCCACTTTATAGTGGTAGAAACTGGTACGA55680.3031068451629199No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45850.24959498654310128No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43720.2379998432205973No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA37580.20457534556793336No Hit
GTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGGGCTGGGTCCG36240.19728074836034876No Hit
GTATTACTGTGCGTCACCCCCACTTTATAGTGGTAGAAACTGGTACGACGCGGGCCTGTACTTTGACTACTGGGG35080.1909660224194546No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG33250.1810039978747681No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32700.17800994678210277No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT32560.17724782468578795No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA30860.16799348494482236No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA30600.16657811533738057No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG30350.1652171830225327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30260.16472724738918745No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29880.16265863027061866No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28920.1574326501816028No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26900.14643631707763194No Hit
GTCTCCAGCCACTCTGTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC26790.14583750685909885No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTG25600.13935946904042296No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24880.13543998397366105No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24840.1352222348032854No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24700.13446011270697059No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24320.1323914955884018No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG24290.13222818371062006No Hit
CACCTGCACTGTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGG23270.12667557986604072No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22900.12466140004006585No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACACCGTAGCAAC22770.12395371523634495No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG22700.12357265418818754No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22650.12330046772521797No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22570.12286496938446664No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22510.12253834562890316No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22450.12221172187333966No Hit
GGCCTGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT22010.11981648099920739No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAATTACTA21790.11861886056214126No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21750.1184011113917656No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT21610.11763898929545077No Hit
GTACAGGCCCGCGTCGTACCAGTTTCTACCACTATAAAGTGGGGGTGACG21410.11655024344357247No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21370.11633249427319681No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21290.11589699593244548No Hit
GTTTATTACTGTCAACACCGTAGCAACTGGCCTCCTTTTGGCCAGGGGAC21180.11529818571391244No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20810.11328400588793758No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20110.1094733954063635No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCC20070.10925564623598784No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19450.1058805340951651No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19410.10566278492478944No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19310.10511841199885028No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19300.10506397470625636No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACACCGTAGCAACTGG19040.10364860509881457No Hit
GTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTA18910.10294092029509366No Hit
GGCCAAAAGGAGGCCAGTTGCTACGGTGTTGACAGTAATAAACTGCAAAA18720.10190661173580928No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18680.10168886256543364No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18640.10147111339505797No Hit
CCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT18440.10038236754317967No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18420.10027349295799184No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18380.10005574378761618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT20150.054.3626570
GGTATCA14150.044.5188521
CGTATGC1500.043.66128541
TCGTATG1500.043.57916640
ATGCCGT1650.039.92218844
ACGAGAC3650.038.97120722
GAGCGAT3200.038.30888732
CTTATAC3950.037.7261961
GTATCAA37650.037.331081
GTGGTAT4150.035.0919721
CACGAGA4000.034.71264321
TGGTATC4100.034.6882132
CTACCGA22300.034.52543669
ATCTCCG4200.033.85677710
GCCGTCT1750.033.81822246
TATACAC4250.033.4630133
TGAGCGA4200.033.21066331
CCGTCTT1800.032.93505547
CGAGACA4450.032.72978623
CCCACGA4250.032.66805319
Produced by FastQC (version 0.11.5)