FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836976
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA67200.3658186062311103No Hit
GTGTATTACTGTGCGTCACCCCCACTTTATAGTGGTAGAAACTGGTACGA55940.30452221477036173No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47890.26070019423226No Hit
GTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGGGCTGGGTCCG39510.215081743038559No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37120.2020712301086133No Hit
GTATTACTGTGCGTCACCCCCACTTTATAGTGGTAGAAACTGGTACGACGCGGGCCTGTACTTTGACTACTGGGG35660.19412338538990168No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA32310.17588689237094007No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT32180.17517920756721916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30910.16826567140779194No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA30780.16755798660407104No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCGGTAGACACGTCCAAGAA30560.16636036616700492No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29270.15933795542238982No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC29200.15895689437423244No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28420.15471078555190707No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28180.1534042905296531No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27970.15226110738518087No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27550.14997474109623643No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26970.14681737812578935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26880.1463274424924441No Hit
GTCTCCAGCCACTCTGTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC26140.1422990828404944No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTG25410.13832516048113858No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24810.13505892292550364No Hit
CACCTGCACTGTCTCTGGTGGCTCCTTCAGCAGTAGTAATTACTACTGGG24590.13386130248843753No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24570.13375242790324968No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24430.13299030580693488No Hit
GGCCTGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGT23020.12531464755119284No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACACCGTAGCAAC22320.12150403706961876No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22260.12117741331405527No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22220.1209596641436796No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT21980.11965316912142564No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGACCCAGCCCCAGTAGTAATTACTA21980.11965316912142564No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21230.115570372176882No Hit
GTACAGGCCCGCGTCGTACCAGTTTCTACCACTATAAAGTGGGGGTGACG20920.11388281610647064No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20570.11197751086568361No Hit
GTTTATTACTGTCAACACCGTAGCAACTGGCCTCCTTTTGGCCAGGGGAC20470.11143313793974445No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20330.11067101584342964No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACACCGTAGCAACTGG19820.10789471392113996No Hit
GTCTCTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCTACTTAGCCTGGTA19750.10751365287298255No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19610.10675153077666774No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19600.10669709348407383No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19270.10490066282847461No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19190.10446516448772329No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19120.1040841034395659No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19120.1040841034395659No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18920.10299535758768757No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG18790.10228767278396668No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18790.10228767278396668No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18720.10190661173580928No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18680.10168886256543364No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18650.10152555068765189No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18430.10032793025058576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGAT20750.056.13826470
TACGTAT1801.8189894E-1244.49152470
GGTATCA15700.044.226111
CGGGATA603.3764263E-739.4975517
CTAACGC350.001480822538.6819953
TGGTATC5050.036.1994632
CTACCGA22350.035.92340569
GTGGTAT4950.035.581371
GTATCAA39300.035.077321
ATCAACG44150.031.0485463
TATCAAC44400.030.8033642
TCAACGC44500.030.72414
GTACGTA1651.4006218E-1030.07071569
AACGCAG45850.030.0408956
CAACGCA45750.029.9586165
TCTACCG25150.028.80383968
AATGGTA6400.028.43918470
ACGCAGA48750.028.2538497
CGCAGAG48950.028.2075448
AGAGTAC49550.027.72938311
Produced by FastQC (version 0.11.5)