FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993259
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACTCAGGCTGCTATCCCATGTT28600.2879410103507746No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27790.2797860376800009No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22450.22602362525786326No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21740.21887543933656778No Hit
GTAATAATGGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG18760.18887319420211646No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC18540.18665826335326435No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16660.16773067246307358No Hit
ATGTATTACTGTGCGAAAGTGTTCACTGTCTCTTCGTTGACCCCTAACTT14840.14940715362256973No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14680.14779629482340456No Hit
CATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTGTGGTATTCGG14650.1474942587985611No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13710.13803046335346572No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGTAA13300.13390263768060495No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13300.13390263768060495No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13290.13380195900565714No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13070.131587028156805No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12900.12987549068269202No Hit
GTAATACATAGATGTGTCCTCAGTTCTCAGGCTGTCCATCTGTAGATACA12710.1279625958586834No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12500.1258483436847791No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12030.1211164459622314No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11710.11789472836390105No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACTCAGG11420.11497504679041418No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11200.11276011594156207No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11180.11255875859166643No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11060.11135061449229254No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11040.1111492571423969No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10930.11004179171797085No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10520.1059139660451101No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG10410.10480650062068403No Hit
GTTTGGGGGCTGTTCCTGGGACTTGCTGGTACCAGGATAGCCAATTATTA10190.10259156977183191No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10140.10208817639709279No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGTAATAATTGGCTATCCTGGTACCAGCAAGTCCCAGGAACAGCCCC10120.10188681904719715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)