FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993259
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37260.37512874285558956No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27610.2779738215309401No Hit
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGCACTCAGGCTGCTATCCCATGTT21820.2196808687361504No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG21080.21223064679001144No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20940.21082114534074192No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20800.2094116438914724No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCAC16580.166925243063491No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15150.15252819254595226No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC15040.1514207271215262No Hit
ATGTATTACTGTGCGAAAGTGTTCACTGTCTCTTCGTTGACCCCTAACTT14730.14829968819814368No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14570.1466888293989785No Hit
GTAATAATGGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG14340.14437321987517857No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14250.14346711180064817No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13760.1385338567282048No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13360.13450670973029188No Hit
CATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCTGTGGTATTCGG13340.13430535238039626No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGTAA13250.13339924430586583No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12690.12776123850878773No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12360.12443884223550956No Hit
GTAATACATAGATGTGTCCTCAGTTCTCAGGCTGTCCATCTGTAGATACA12110.121921875361814No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11170.1124580799167186No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10940.11014247039291866No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10780.10853161159375349No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG10700.1077261821941709No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10360.10430310724594491No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG9990.10057799627287546No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9960.10027596024803198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG400.006637892753.1126770
GGTATCA10450.042.3354761
TGGTATC3500.037.9090352
GTATCAA25800.037.5926171
ATAGCGG1900.032.17715563
ATCAACG30050.032.0332223
TCAACGC30250.031.8182284
TATCAAC30500.031.4554122
CAACGCA30550.031.2846955
AACGCAG31100.030.9501576
TACGAAA950.005299930529.81763670
TAGCGGC2100.029.20908464
AATAGCG2100.029.02612162
CGCAGAG34750.027.7971538
CGATAGA500.00790882527.47412937
TAACGGC1005.260499E-727.45040336
TCTCGCG3103.8198777E-1127.4129970
AGAGTAC35450.027.24689511
GCAGAGT35850.027.1339919
CAGAGTA35900.027.00145710
Produced by FastQC (version 0.11.5)