FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989021
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34180.34559427959568095No Hit
GTATAATAATTGGCCCCCGGAGACCTTCGGCCAAGGGACGCGACTGGAAATTAAACGAACTGTGGCTGCACCAT33910.34286430722906797No Hit
TTATTATACTGGTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC30250.30585801514831334No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28790.2910959423510724No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA26890.27188502569712875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24330.246000843258131No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23800.24064200861255727No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22350.2259810459029687No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCACCAGTATAATAATTGG19720.19938909285040463No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGCGGGTCTGGGACAGAGTTCACT19030.19241249680239347No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18620.18826698320864774No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18300.18503146040377302No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17950.1814926073359413No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17500.1769426533915862No Hit
GAGTATTAGCAGGAACTTAGCCTGGTACCAACAAAAAGTTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATC16790.16976383716827043No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGGAACTTAGCCTGGTACCAACAAAAAGTTGGCCAGGCTCCCAGGCT16760.16946050690531345No Hit
GTTTATTACTGTCACCAGTATAATAATTGGCCCCCGGAGACCTTCGGCCA16520.16703386480165738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15690.1586417275265136No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15460.1563161955105099No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15120.1528784525303305No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15110.15277734244267815No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15040.15206957182911182No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14790.14954181963780344No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGGAACTTAGCCTGGTA14410.1456996363070147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14300.14458742534283903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14300.14458742534283903No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14150.14307077402805402No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13990.14145301262561666No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13870.14023969157378863No Hit
GGATACCAGTGGCCCTGGTGGATGAACCATAGATGAGGAGCCTGGGAGCCTGGCCAACTTTTTGTTGGTACCAGG13180.1332630955257775No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGCGTCC13070.13215088456160182No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12550.12689316000368042No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGCGTCCCTTGGCCGAAGGTC12540.12679204991602808No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12520.1265898297407234No Hit
CATCTATGGTTCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGCGGGTCTGGGACAGAGTTCA12270.12406207754941502No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG11980.1211298850074973No Hit
GTATTAGCAGGAACTTAGCCTGGTACCAACAAAAAGTTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATCCA11760.11890546307914594No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC11550.11678215123844692No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA11390.11516438983600955No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11220.11344551834591984No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11190.11314218808296285No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11180.11304107799531052No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA10970.11091776615461148No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAACTTTTTGTTGGTACCAGGC10910.11031110562869748No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10870.10990666527808814No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10720.10839001396330311No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10260.1037389499312957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA200.002416885568.5515169
CGCGTAT252.6025012E-455.0466139
GACGTCA400.006026380754.42601870
ATCGCGT408.7499757E-751.32320437
ATTCCGT200.00812657550.46685829
GCGCATA200.00832776150.1555869
TTACGCG406.515941E-445.70100869
ACGAGAC4850.042.0952922
CCTATAC406.010224E-541.802663
GTAGTTT1053.794983E-641.4674470
CCCACGA5050.041.0701219
CACGAGA5000.040.8200421
TCTCCGA5100.040.6489711
CTTATAC5100.040.0054781
TATACAC5700.039.895873
ATCTCCG5200.039.8672610
CGCGGAT350.001329973139.53494341
TGTTAGA11250.039.34799670
GTATCAA27600.038.7793661
CGAGCCC5350.038.75340715
Produced by FastQC (version 0.11.5)