FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989021
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45960.46470196285013154No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35940.36338965502249193No Hit
GTATAATAATTGGCCCCCGGAGACCTTCGGCCAAGGGACGCGACTGGAAATTAAACGAACTGTGGCTGCACCAT33670.3404376651254119No Hit
TTATTATACTGGTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC33180.3354832708304475No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25220.2549996410591888No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24720.2499441366765721No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA23680.23942868756072927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23660.23922646738542458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20460.2068712393366774No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCACCAGTATAATAATTGG19810.2002990836392756No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19340.19554690951961587No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17440.17633599286567223No Hit
GTCCCAGACCCGCTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT17260.17451601128793018No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGCGGGTCTGGGACAGAGTTCACT16900.17087604813244614No Hit
GAGTGAACTCTGTCCCAGACCCGCTGCCACTGAACCTGGCTGGGATACCA16310.16491055296095838No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15930.16106836963016963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15330.15500176437102953No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15320.1549006542833772No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14850.15014848016371746No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14760.14923848937484643No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14590.14751961788475673No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA14490.1465085170082334No Hit
GAGTATTAGCAGGAACTTAGCCTGGTACCAACAAAAAGTTGGCCAGGCTCCCAGGCTCCTCATCTATGGTTCATC14420.14580074639466706No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14370.14529519595640536No Hit
GTTTATTACTGTCACCAGTATAATAATTGGCCCCCGGAGACCTTCGGCCA14300.14458742534283903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14270.14428409507988202No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGGAACTTAGCCTGGTACCAACAAAAAGTTGGCCAGGCTCCCAGGCT14120.142767443765097No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGCGTCCCTTGGCCGAAGGTC14040.14195856306387833No Hit
GGATACCAGTGGCCCTGGTGGATGAACCATAGATGAGGAGCCTGGGAGCCTGGCCAACTTTTTGTTGGTACCAGG13770.1392285906972653No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13580.13730749903187092No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13530.13680194859360925No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13380.1352852972788242No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13220.13366753587638686No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGCGTCC13100.13245421482455882No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12640.12780315079255142No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12210.12345541702350102No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12030.12163543544575899No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11900.12032100430627864No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAGGAACTTAGCCTGGTA11870.12001767404332163No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11260.1138499586965292No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10940.11061443589165448No Hit
CATCTATGGTTCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGCGGGTCTGGGACAGAGTTCA10770.10889556440156478No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10660.1077833534373891No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10400.1051544911584284No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10240.10353672975599103No Hit
CCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTC10110.10222229861651068No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10090.102020078441206No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10010.10121119773998732No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9920.1003012069511163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACC1102.3646862E-1134.92370245
GGTATCA11850.034.6704861
GTATCAA29250.034.258521
GCTATAC400.003052008333.3800933
CTAGTTG400.003052008333.3800934
TGCACAG3850.030.97096370
GCGGTAC1251.10958354E-1030.65177244
CCCTTAT553.936487E-430.3655051
TATCAAC32950.030.1905042
ATCAACG33750.029.5722923
TCAACGC34100.029.1708744
ACGGGTA1501.8189894E-1228.94405416
AACGCAG34800.028.8718626
CAACGCA34500.028.8326635
TTAACGG1302.3101165E-1028.79514535
AACGGCC1202.9504008E-928.4969637
TAACCGT1403.0559022E-1028.11084653
AGAGTAC35550.027.79327211
CGCAGAG36000.027.6313028
ATTAGAG1950.027.3887963
Produced by FastQC (version 0.11.5)