FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072819
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36460.3398522956808185No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30010.27973031797535275No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24770.23088703686269538No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA24690.23014133791441055No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24550.22883636475491206No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22780.21233777552410985No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21940.20450793656711896No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT21930.20441472419858336No Hit
TTATTACTGTCAGCAGTATTCTTACTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG21110.19677130997866366No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20290.18912789575874403No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20170.18800934733631675No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19440.18120484443321755No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19420.18101841969614632No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18760.17486640337279633No Hit
CCATAGATGAGGAGCCTGGGAGACTGCCCAGGTCTCTGCTGGTACCAGGC17730.16526552941362896No Hit
GGCCAAAACTGTACCAGTAAGAATACTGCTGACAGTAATAAAGTGCAAAA17060.1590203007217434No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT16640.15510538124324794No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16550.15426646992642748No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16190.15091082465914568No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16000.14913978965696914No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15810.14736875465479266No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15680.14615699386382977No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT15310.14270813622801237No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT15240.14205564964826312No Hit
CAGTAATAAAGTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15220.14186922491119192No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15170.1414031630685139No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15160.1413099506999783No Hit
GTAAGAATACTGCTGACAGTAATAAAGTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT15080.14056425175169343No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14860.13851357964391012No Hit
GTATTCTTACTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14650.1365561199046624No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14590.13599684569344875No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14330.133573324111523No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCACTTTA14090.13133622726666846No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGACAGCCACTTAGCCTGGTA13880.12937876752742075No Hit
GACCTGGGCAGTCTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCC13380.12471814910064045No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13230.12331996357260637No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13140.12248105225578591No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13040.12154892857042987No Hit
CTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCA12780.11912540698850412No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12760.11893898225143291No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12260.11427836382465262No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12250.114185151456117No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12160.11334624013929655No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11910.11101593092590642No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11630.10840598460690946No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGACAG11490.10710101144741097No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11400.10626210013059052No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA11090.10337251670598675No Hit
CTTTATTACTGTCAGCAGTATTCTTACTGGTACAGTTTTGGCCAGGGGAC11070.10318609196891554No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11020.10272003012623751No Hit
GGAGAAATAGTGATGACCCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG10880.10141505696673903No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG10830.10094899512406101No Hit
GAGTGTTGACAGCCACTTAGCCTGGTACCAGCAGAGACCTGGGCAGTCTCCCAGGCTCCTCATCTATGGTGCGTC10820.1008557827555254No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10750.10020329617577614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT501.1477823E-955.02948839
CGCTCGA200.0082469950.27971314
CGTATGC2350.047.03378741
TCGTATG2100.045.95680640
AATCGCG306.6518557E-445.51185236
ACGAGAC4800.041.9349422
CTTATAC5150.041.659571
CGAGACA5000.040.261323
ATGCCGT3000.039.4718944
GGTATCA12050.039.2260061
AATGTCG1050.039.0101636
TATACAC5700.038.2243423
CCCACGA5550.038.06725719
ATGTCGT1001.0913936E-1137.62763637
ATCTCCG5650.036.782810
CGAGCCC5700.036.4618415
TCCGAGC5700.036.4601413
GCCCACG5800.036.42302718
TCTCCGA5750.036.14309711
CCGAGCC6000.035.19579714
Produced by FastQC (version 0.11.5)