FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072819
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36550.34069120699763894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36100.33649665041353666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26800.24980914767542334No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26270.24486889214303625No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA24070.22436217106520298No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23460.218676216584531No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATT22180.2067450334119735No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21120.19686452234719928No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20680.19276317813163266No Hit
CATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20100.1873568607565675No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA19840.18493333917464175No Hit
TTATTACTGTCAGCAGTATTCTTACTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG19500.18176411864443118No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18870.175891739426688No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18280.17039220968308727No Hit
GGCCAAAACTGTACCAGTAAGAATACTGCTGACAGTAATAAAGTGCAAAA18100.16871438704944638No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17090.1592999378273502No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17070.15911351309027896No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17020.15864745124760093No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16650.15519859361178354No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16330.15221579781864414No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT15960.14876694018282674No Hit
CCATAGATGAGGAGCCTGGGAGACTGCCCAGGTCTCTGCTGGTACCAGGC15700.146343418600901No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15420.14373347228190403No Hit
CAGTAATAAAGTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15340.1429877733336192No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15330.14289456096508357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15040.14019140227755103No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14800.1379543054326965No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT14600.13609005806198435No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14230.13264120042616695No Hit
GTAAGAATACTGCTGACAGTAATAAAGTGCAAAATCCTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAGT14100.13142943963520407No Hit
GTATTCTTACTGGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14080.13124301489813286No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13850.12909913042181395No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13820.12881949331620712No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCTGAGGATTTTGCACTTTA13760.1282602191049935No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13550.12630275936574575No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGAGTGTTGACAGCCACTTAGCCTGGTA13500.12583669752306773No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13180.12285390172992834No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13090.1220149904131079No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12490.11642224830097155No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11890.1108295061888352No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11710.1091516835551943No Hit
GACCTGGGCAGTCTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCC11670.10877883408105188No Hit
CTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCA11670.10877883408105188No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11270.10505033933962764No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11200.10439785275987842No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT11180.10421142802280721No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11090.10337251670598675No Hit
GGAGAAATAGTGATGACCCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG11050.10299966723184434No Hit
GAGTGTTGACAGCCACTTAGCCTGGTACCAGCAGAGACCTGGGCAGTCTCCCAGGCTCCTCATCTATGGTGCGTC10830.10094899512406101No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10810.1007625703869898No Hit
GCAGAGACCTGGGCAGTCTCCCAGGCTCCTCATCTATGGTGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTT10790.10057614564991858No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10780.10048293328138297No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA10740.10011008380724055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)