FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252960
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACTACTATGATACTTCATCCACCTTCGGCCAAGGGACACGACTGGAAATGAAACGAACTGTGG34160.2726344017366875No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25450.20311901417443493No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23510.18763567871280806No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC22720.1813306091176095No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19400.15483335461626868No Hit
GTGTATTACTGTCACTACTATGATACTTCATCCACCTTCGGCCAAGGGAC18230.14549546673477207No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18190.14517622270463543No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17670.14102605031285914No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17020.1358383348231388No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16370.13065061933341846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15980.12753799003958627No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15080.12035499936151194No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14840.11843953518069213No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCC14520.11588558293959904No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14180.11317200868343762No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13820.1102988124122079No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13600.10854297024645639No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13370.10670731707317074No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13030.10399374281700932No Hit
CTATGATACTTCATCCACCTTCGGCCAAGGGACACGACTGGAAATGAAACGAACTGTGGCTGCACCATCTGTCTT12830.10239752266632614No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12810.10223790065125782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2500.048.40406441
TCGTATG2600.045.10396240
ATGCCGT2750.044.2584344
GGTATCA19800.042.171771
GCCGTCT2750.039.36030646
CCCACGA4000.038.02990319
ATCTCGT2850.037.2935737
CTAACGC558.506171E-636.876013
ACGAGAC4050.036.73745322
CTTATAC4250.036.5897061
GTATGCC3450.036.13804642
CACGAGA4050.035.89964321
CGAGCCC4350.034.9658415
TATACAC4850.034.8484653
TCTCCGA4400.034.568511
CCGTCTT3050.034.385147
GTATCAA51700.034.0018731
CTACATC3250.033.56291233
ATCTCCG4400.033.03212410
CATCTCG3350.031.67735936
Produced by FastQC (version 0.11.5)