FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252960
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACTACTATGATACTTCATCCACCTTCGGCCAAGGGACACGACTGGAAATGAAACGAACTGTGG34670.2767047631209297No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31040.24773336738602986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25530.20375750223470823No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21690.17311007534159112No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCC19040.15196015834503895No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19010.15172072532243647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19010.15172072532243647No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18970.15140148129229983No Hit
GTGTATTACTGTCACTACTATGATACTTCATCCACCTTCGGCCAAGGGAC18840.15036393819435578No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18650.14884752905120674No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15630.1247446047758907No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15470.12346762865534415No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15200.12131273145192184No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14510.11580577193206487No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14150.11293257566083516No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13460.10742561614097816No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13000.10375430979440683No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12820.10231771165879197No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTAATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCC12740.10167922359851869No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12730.10159941259098455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.040.1524541
GTATCAA51550.036.509171
ATTCGAT750.00202278736.29820370
CGATCTA400.00271641234.18355635
ATCAACG55650.033.6764143
TATCAAC55900.033.4734152
TCAACGC56700.033.111014
CAACGCA57900.032.4856835
AACGCAG58450.032.4110956
CGCAGAG61800.030.6541828
ACGCAGA62450.030.443277
TACGTAT1802.2474887E-730.248570
AGAGTAC62400.029.98061411
GCAGAGT64900.028.9818349
CAGAGTA67150.027.91016210
ACGGGTA4850.026.4675216
AATGGTA11150.026.24701970
TTAACGG1209.314317E-825.63766735
GAGTACT30300.025.51958312
GTACGTA1801.2574674E-824.8716269
Produced by FastQC (version 0.11.5)