FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926714
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA42940.4633576270564597No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30560.3297673284314255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29210.31519972720817857No Hit
GTATAAAACTCTTTACAGTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT23910.25800840388728347No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20600.22229080385102631No Hit
GTTTTATACTGCTGGCAGTAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGTATTCTGTC19460.2099892739291734No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19240.2076152944705702No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17860.19272396877569564No Hit
GAGTATTGATGGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGACAT16920.18258060199802742No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG16850.1818252448975628No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGTATTGATGGCTGGTTGGCCTGGTATCAGCAGAAA16690.180098714382215No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16680.17999080622500577No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA16110.17384004126407931No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15870.17125024549105766No Hit
GAGTGTATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA15640.16876835787524522No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15390.16607065394501433No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15140.1633729500147834No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14850.16024361345571556No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA14540.15689846058222925No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14440.1558193790101369No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14320.15452448112362605No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13580.1465392774901426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13430.14492065513200406No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT13250.14297830830223782No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13010.14038851252921614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12930.13952524727154225No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12610.1360721862408467No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12070.13024514575154794No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11990.12938188049387406No Hit
TCAATACTCTGACTGGCCCGGCAAGTGATAGTGACTCTGTCTCCTACAGA11870.12808698260736323No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11550.12463392157666767No Hit
GTGTATTACTGTGCGAGAAATCTCGGCGGTAACTACTACAACTACTTCTT11430.12333902369015683No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11300.12193621764643678No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11070.11945433003062433No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10870.11729616688643961No Hit
GTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCA10860.11718825872923037No Hit
GGCCAAAACTGTAAAGAGTTTTATACTGCTGGCAGTAGTAAGTTGCAAAA10760.116109177157138No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10410.11233239165481476No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10150.10952677956737461No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10030.10823188168086377No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9910.10693698379435296No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9840.10618162669388831No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9760.10531836143621441No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9640.10402346354970357No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9520.10272856566319274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACA350.00467794358.0236270
AGTTACG306.6707307E-445.4851042
TAACGAT5500.044.3089570
ACCGGAC350.001131277540.85720462
TATACAC2200.040.316343
ACGAGAC1800.039.8123622
GTGGTAT2600.039.3642351
CTAACGC350.001424349138.9872323
GTATCAA21200.037.495051
ATCTCGT1300.037.0277537
CGAGACT1950.035.00176623
TCGTATG1300.034.48420740
CTTATAC2500.034.115671
GTAGTTT1651.0972326E-732.8214470
TCAACGC24350.032.64284
ATCAACG24450.032.5092933
TATCAAC24450.032.3697662
GCTAATC1700.032.27435733
CACGAGA2250.031.84472821
ATACACA2700.031.5868784
Produced by FastQC (version 0.11.5)