FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences926714
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATACACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA39210.42310788441741465No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32150.346924725427694No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24200.2611377404463513No Hit
GTATAAAACTCTTTACAGTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT22640.2443040679217105No Hit
GAGTGTATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA22000.23739794586031937No Hit
GTTTTATACTGCTGGCAGTAGTAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGTATTCTGTC20990.2264992219821865No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20760.22401733436637408No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18870.20362269265382849No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17700.19099743826034785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17690.1908895301031386No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17090.18441504067058445No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA16290.17578238809384555No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG15640.16876835787524522No Hit
GAGTATTGATGGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATAAGACAT15540.16768927630315286No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15500.16725764367431592No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15290.16499157237292197No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGTATTGATGGCTGGTTGGCCTGGTATCAGCAGAAA14390.15527983822409072No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA13690.14772626721944418No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13650.14729463459060724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13540.14610764486130565No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13260.14308621645944705No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13180.14222295120177314No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13100.14135968594409926No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG13020.14049642068642537No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12790.13801453307061293No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12330.13305075783898807No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12290.13261912521015112No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11970.1291660641794556No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11800.12733162550689858No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11700.1262525439348062No Hit
GTGTATTACTGTGCGAGAAATCTCGGCGGTAACTACTACAACTACTTCTT11640.1256050949915508No Hit
TCAATACTCTGACTGGCCCGGCAAGTGATAGTGACTCTGTCTCCTACAGA11540.12452601341945843No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11230.12118086054597212No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11000.11869897293015969No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10920.11783570767248579No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10900.11761989135806732No Hit
GTTTTGGCCAGGGGACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCA10100.10898723878132843No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9880.10661325932272525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9720.10488672880737747No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9480.10229693303435579No Hit
GGCCAAAACTGTAAAGAGTTTTATACTGCTGGCAGTAGTAAGTTGCAAAA9350.10089412699063573No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9280.10013876989017109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA200.005595945855.45921367
TAACGAT5600.043.4003270
GTATCAA18350.041.767961
GTATGCG350.001436121938.92198616
GTGGTAT1850.036.8260461
TATCAAC21800.034.6685332
TCAACGC22450.033.3686834
ATCAACG22600.033.1454163
CTAGACT652.53832E-531.4318984
ACTCTTT22900.030.92983270
ACGCAGA24650.030.6651677
CGCAGAG24800.030.6169878
AGAGTAC25050.030.58327911
CAACGCA24850.030.4200065
AACGCAG25000.030.2374886
CTAACGA5750.029.52407569
GCAGAGT26200.028.9809679
TGTGTCG6750.028.8049570
GTAGTTT1755.271042E-627.77620370
CAGTTCG12050.027.08467970
Produced by FastQC (version 0.11.5)