FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230579
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46810.3803900440361813No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44820.36421879456743533No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38830.31554252104090835No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35910.29181385347872835No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC35630.28953850179468366No Hit
TCTCTGGACTCCAGGTTGAAGACGAGGCTGATTATTACTGCAGCTCATATACAAGCGACAATACTTATGTCCTCG34620.281330983220094No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34090.277024067532438No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC33080.2688165489578483No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30090.24451904347465706No Hit
GATTATTACTGCAGCTCATATACAAGCGACAATACTTATGTCCTCGCACCTGGGACCACGGTCACCGTCCTGGGT30010.24386894299350143No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29610.24061844058772333No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26400.21453315878135412No Hit
GCCTTGGGCTGACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGTCGCTTGTATATGAGCTG26230.21315169525889846No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25000.20315640036113083No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCTCTGGGCTCACCAGTGACCTT24360.19795559651188585No Hit
CTCCTGCTCTGGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTACCAACAACACCCAGGCAAAGT23780.19324236802350764No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23160.1882040892945516No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGAAGCCGGAGAAG22910.18617252529094028No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22250.18080919632140643No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21960.17845258207721731No Hit
GTAATAATCAGCCTCGTCTTCAACCTGGAGTCCAGAGATGGTCAGGGAGG21450.17430819150985025No Hit
ACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGTCGC20600.1674008738975718No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20510.1666695108562717No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGT20050.1629314330896269No Hit
CCTTAATCCCTATAACTTTATCTCCTGGTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGT19070.15496770219547057No Hit
GTCCCCAAACTCATCATTTATGAGGTCAGTAATCGGCCCCCAGGGGTTTCTGATCGCTTCTCCGGCTTCAAGT18460.15001068602665898No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18440.14984816090637007No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18150.14749154666218098No Hit
GTCGATCACCATCTCCTGCTCTGGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTACCAACAACA18130.14732902154189206No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17410.1414781172114915No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCAC17150.13936529064773573No Hit
ATACTTATGTCCTCGCACCTGGGACCACGGTCACCGTCCTGGGTCAGCCC16360.13294554839632403No Hit
GACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGTCGCTTGTATATGAGCTGCAGTAATAAT15670.1273384317463568No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15620.12693211894563453No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGATAAAGTTATAG15590.12668833126520118No Hit
GACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCACCATCT15430.12538813030288995No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCTTCAACCTGGAGTCCAGA15370.12490055494202322No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14760.11994353877321164No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14620.1188058629311893No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14440.11734313684858914No Hit
GCTCTGGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTAC14440.11734313684858914No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14180.1152303102848334No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC14030.1140113718826666No Hit
ACTTATGTCCTCGCACCTGGGACCACGGTCACCGTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC13670.11108591971746634No Hit
GACATAAGTATTGTCGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCTTCAACCTGGAGTCCAGAGATGGT13430.10913561827399947No Hit
CATAAGTATTGTCGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCT13390.10881056803342168No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT13350.10848551779284385No Hit
GTATTGTCGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCTTCAAC13210.10734784195082152No Hit
GCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCAC13170.10702279171024372No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12950.10523501538706576No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12820.10417860210518788No Hit
GGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTACCAACA12810.10409733954504342No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12560.10206577554143213No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12450.10117188737984315No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC12410.10084683713926534No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12380.10060304945883197No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12360.10044052433854309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTT200.007418417451.64509239
CGCGTAT200.007418417451.64509239
TACGTAT554.1025446E-450.19556470
ACTCGAC3100.048.41301727
CGTATGC1500.048.3610241
GGTATCA14750.047.3423841
CTCGACG3250.047.2453928
GACTCGA3250.047.19927226
TCGACGT3050.047.01593429
TCGTATG1400.046.80919640
ATCGCGT306.429422E-445.8276737
CTTATAC3500.043.7958341
TATACAC3700.042.3473973
ACGAGAC3700.042.34739722
AGACTCG3750.041.7997625
CGACGTC3450.041.59700830
CTAACGC504.19571E-640.874453
GACGACG4750.040.68482670
CACGAGA3950.040.52622221
GCGTATG350.001349580539.4182740
Produced by FastQC (version 0.11.5)