FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230579
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42210.3430092663697333No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40390.32821948042344296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38060.30928530390978554No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34870.28336254722370524No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34310.2788118438556159No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32750.26613488447308137No Hit
TCTCTGGACTCCAGGTTGAAGACGAGGCTGATTATTACTGCAGCTCATATACAAGCGACAATACTTATGTCCTCG31860.2589025166202251No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31760.2580898910187806No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30500.2478508084405796No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29070.23623026233992292No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27810.22599117976172192No Hit
GATTATTACTGCAGCTCATATACAAGCGACAATACTTATGTCCTCGCACCTGGGACCACGGTCACCGTCCTGGGT26850.2181899739878545No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25690.20876351701109805No Hit
GCCTTGGGCTGACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGTCGCTTGTATATGAGCTG25320.20575680228575327No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25300.20559427716546436No Hit
CTCCTGCTCTGGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTACCAACAACACCCAGGCAAAGT23270.18909797745614054No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22950.18649757553151808No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22550.18324707312574No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGAAGCCGGAGAAG21200.17227662750623893No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCTCTGGGCTCACCAGTGACCTT21130.17170778958522775No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21090.17138273934464995No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21060.17113895166421658No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGT20820.16918865022074975No Hit
GTAATAATCAGCCTCGTCTTCAACCTGGAGTCCAGAGATGGTCAGGGAGG20200.1641503714917937No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCAC20130.16358153357078253No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19120.15537401499619286No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18650.1515546746694036No Hit
GTCCCCAAACTCATCATTTATGAGGTCAGTAATCGGCCCCCAGGGGTTTCTGATCGCTTCTCCGGCTTCAAGT18140.14741028410203652No Hit
ACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGTCGC18120.1472477589817476No Hit
GTCGATCACCATCTCCTGCTCTGGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTACCAACAACA17340.14090927929048033No Hit
CCTTAATCCCTATAACTTTATCTCCTGGTACCAACAACACCCAGGCAAAGTCCCCAAACTCATCATTTATGAGGT16850.13692741384340218No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16800.1365211010426799No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA16070.1305889341521349No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15860.1288824203891014No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15440.12546939286303438No Hit
GACCCAGGACGGTGACCGTGGTCCCAGGTGCGAGGACATAAGTATTGTCGCTTGTATATGAGCTGCAGTAATAAT15290.1242504544608676No Hit
ATACTTATGTCCTCGCACCTGGGACCACGGTCACCGTCCTGGGTCAGCCC14950.12148752741595623No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14890.12099995205508951No Hit
GACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCACCATCT14470.11758692452902252No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14230.11563662308555567No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14120.11474273492396668No Hit
GCCCTGACTCAGCCTGCCTCCGTTTCTGGGTCTCCTGGACAGTCGATCAC14010.11384884676237772No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCTTCAACCTGGAGTCCAGA13970.11352379652179989No Hit
GGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTACCAACA13760.1118172827587664No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13400.10889183059356612No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGATAAAGTTATAG13370.10864804291313276No Hit
GCTCTGGGCTCACCAGTGACCTTAATCCCTATAACTTTATCTCCTGGTAC13350.10848551779284385No Hit
GTATTGTCGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCTTCAAC13280.10791667987183268No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13220.10742910451096598No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13110.106535216349377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)