FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1353870
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTACACTGAGAATCAGCAGAGTGGAGTCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACT95420.7047944041894716No Hit
GTGTAAACTCTGTGCCTCTAACACTGCCACTGAACCTGTCAGGGACCCCG79950.5905293713576636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74720.5518993699542792No Hit
GTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAGGCTGGATATCAAG56380.4164358468686063No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49980.3691639522258415No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCTTG48150.3556471448514259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47100.3478915996365973No Hit
GGTCTAGTCAGAGCCTCTTGCATCCTAATGGAAAAACATTTTTCGATTGGTACCTGCAGAAGCCAGGGCAGTCTC42490.31384106302673076No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42350.31280699033142034No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGACTCCACTCTGCTGATTCTCAGTGTAAACT40980.30268785038445345No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40710.3006935673292118No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38730.28606882492410646No Hit
GTAATAAACCCCAACATCCTCAGACTCCACTCTGCTGATTCTCAGTGTAAACTCTGTGCCTCTAACACTGCCA38280.28274501983203704No Hit
GTGTTAGAGGCACAGAGTTTACACTGAGAATCAGCAGAGTGGAGTCTGAG37590.27764851869086393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32680.24138211201961784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31700.23414360315244445No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31410.23200159542644422No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30570.22579715925458133No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30010.22166086847333938No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT29570.2184109257166493No Hit
GTGCAGCCACAGTTCGCTTGATATCCAGCCTGGTCCCCTGGCCAAAAGTG29480.21774616469823543No Hit
CTTTTGGCCAGGGGACCAGGCTGGATATCAAGCGAACTGTGGCTGCACCA29410.2172291283505802No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATATCCAGCCTGGTCC28620.2113940038556139No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28400.20976903247726886No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT27390.20230893660395755No Hit
GGCCAAAAGTGTACGGAGTTTGTAGACCTTGCATGCAGTAATAAACCCCA27080.2000192042071986No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26850.19832037049347429No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26350.19462725372450826No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAACCCCAACATCCTCAGACTCCACTCTGCTGATTCTCAGTGTAAACT26260.1939624927060944No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26230.19374090569995644No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25950.19167276030933544No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25570.1888659915649213No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25090.18532059946671395No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24240.17904230095947174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24110.17808209059954055No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGA24080.1778605035934026No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC23990.17719574257498874No Hit
GATCAGGACCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCGAAAA23520.17372421281216072No Hit
AGCCAGGGCAGTCTCCACAGGTCCTGATCTACTCGGGCTCTATTCGGGCC23150.17099130640312585No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA23090.17054813239084995No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAGGCTGGATATCAAGCGAA22910.1692186103540222No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG22760.16811067532333238No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAGGCTGGATATCAAGCG22470.1659686675973321No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACTCTCC22020.1626448625052627No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21280.157179049687193No Hit
GTCCCTGACAGGTTCAGTGGCAGTGTTAGAGGCACAGAGTTTACACTGAG21070.1556279406442273No Hit
CTATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGTTAGAGGC21060.15555407830884796No Hit
GTCCTGATCTACTCGGGCTCTATTCGGGCCTCCGGGGTCCCTGACAGGTT20810.15370751992436496No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTCCACT20360.15038371483229557No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20260.14964509147850236No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20040.14802012010015733No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20030.14794625776477802No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19790.14617356171567433No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19510.1441054163250534No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19350.14292361895898426No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18810.13893505284850097No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18740.13841801650084573No Hit
GTTCAGTGGCAGTGTTAGAGGCACAGAGTTTACACTGAGAATCAGCAGAG18510.13671918278712136No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18250.13479876206725905No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCGTACACTTTTGGCCAGGGGA18200.13442945039036244No Hit
GTCAGGGACCCCGGAGGCCCGAATAGAGCCCGAGTAGATCAGGACCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18150.13406013871346584No Hit
AGTCAGAGCCTCTTGCATCCTAATGGAAAAACATTTTTCGATTGGTACCT17760.13117950763367237No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17740.1310317829629137No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATTTCC17690.13066247128601713No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT17300.12778184020622363No Hit
ATTCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGTTAGAGGCAC17060.12600914415711997No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16700.12335010008346443No Hit
GGTCCTGATCTACTCGGGCTCTATTCGGGCCTCCGGGGTCCCTGACAGGT16690.12327623774808512No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16510.12194671571125736No Hit
GCCCTGGCTTCTGCAGGTACCAATCGAAAAATGTTTTTCCATTAGGATGC16310.12046946900367095No Hit
CAGTAATAAACCCCAACATCCTCAGACTCCACTCTGCTGATTCTCAGTGTAAACTCTGTGCCTCTAACACTGCCA16300.12039560666829163No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16180.1195092586437398No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16130.11913994696684321No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15890.1173672509177395No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15760.11640704055780836No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAAATTGTGATGACTCAGTCTC15580.1150775185209806No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15550.11485593151484264No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15490.11441275750256673No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15450.11411730816104942No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT15440.11404344582567011No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGGTCCTGATCTACTCGGGCTCTATTCGGGCCTCCGGGGTCCCTGA15410.11382185881953215No Hit
CTCCACAGGTCCTGATCTACTCGGGCTCTATTCGGGCCTCCGGGGTCCCT15170.11204916277042848No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG14950.11042419139208344No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGG14930.1102764667213248No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14920.11020260438594548No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14890.10998101737980752No Hit
CCACAGGTCCTGATCTACTCGGGCTCTATTCGGGCCTCCGGGGTCCCTGA14830.10953784336753158No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14750.10894694468449703No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14550.10746969797691064No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14500.10710038630001403No Hit
GTCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAGG14340.10591858893394492No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT14230.1051061032447724No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14020.10355499420180668No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13810.10200388515884096No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13770.10170843581732367No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13610.10052663845125456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)