FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258791
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39070.3103771793729062No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31370.24920737437747806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31200.24785687218926733No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30360.24118380255340244No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27340.21719252838636438No Hit
GTATGATGATCTGCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT24400.19383678466083726No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22980.18255611932401805No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22150.17596249099334202No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21820.1733409279221094No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21640.17191098442870975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21400.17000439310417695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20360.1617424973645347No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19840.15761154949471357No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18400.1461720015475166No Hit
ATATTACTGTCAACAGTATGATGATCTGCCTCTCACCTTCGGCCAAGGGA18280.1452187058852502No Hit
TCATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTGAAATCTGTC17650.14021390365835154No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17320.13759234058711892No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17020.1352091014314529No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16410.13036318181493195No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16400.13028374050974306No Hit
GTCCCAACCAGGACACAGCATGGGCATGGGGGTCCCTGCTCAGCTCCTGG16260.12917156223709894No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAAGTCTTTAAATTGGTATCAACAGAAG16160.12837714918521026No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16120.1280593839644548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15690.12464340784133347No Hit
GTCTGGGACAGATTTCACTTTCACCATCAGCAGCCTGCAGCCTGAAGATA15260.12122743171821217No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT15200.12075078388707895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14990.11908251647811273No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14280.11344218380970313No Hit
ACATATTACTGTCAACAGTATGATGATCTGCCTCTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA13930.11066173812809274No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13590.10796073375167126No Hit
AGTCAGGACATTAGCAAGTCTTTAAATTGGTATCAACAGAAGCCAGGAAA13570.10780185114129351No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTT13520.10740464461534917No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13500.10724576200497145No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13450.1068485554790271No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13360.10613358373232729No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13240.10518028807006087No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13080.10390922718703899No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG12960.10295593152477257No Hit
GTTCAGTGGAGGTGGGTCTGGGACAGATTTCACTTTCACCATCAGCAGCC12930.10271760760920598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT754.4629996E-756.57577570
CGGCCTA200.00756424351.3914738
CCTAACG652.7102942E-1046.6428382
CTAACGC804.5474735E-1142.1064453
CGTGCGT750.001675571437.71718270
GTGGTAT5100.036.3589131
AATGGTA5850.035.0576470
ATCAACG55900.032.901783
AACGCAG56500.032.6107066
TCAACGC56050.032.572044
CAACGCA57000.032.1473665
CGCAGAG60300.030.3321938
GTACGTA1052.67842E-630.305269
GTATCAA62350.030.280981
AGAGTAC61450.030.0386211
CAGAGTA61700.029.80772410
GCAGAGT61950.029.4699429
CGCTAAG500.00574674329.33141963
TATCAAC68800.027.3213122
ACGCAGA68900.026.83957
Produced by FastQC (version 0.11.5)