FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022488
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38470.37623913434680895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36520.35716800588368763No Hit
CTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT34570.33809687742056627No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31300.3061160619977936No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28110.2749176518453028No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25380.248218071996933No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25040.2448928495982349No Hit
CTATCATAGTGTTGACAGTAATATGTTGCAATATCCTCAGGCTGCAGACTGCTGATGGTGAAAGTAAAATCTGTC22870.22367010664183837No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22430.2193668776552879No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22130.21643285789173075No Hit
ATATTACTGTCAACACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGA21740.21261863219910648No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG21110.20645719069563653No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20610.20156715775637465No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20170.19726392876982418No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19450.19022228133728708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18380.17975761084726669No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18110.17711699306006526No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17980.17584558449585716No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17960.1756499831782867No Hit
GCCCCTAACCTCGTGATCTACGATGCATCGAATTTGGAGACAGGAGTCCCATCAAGGTTCAGTGGGAGTGGAT17850.1745741759316491No Hit
ATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA17650.17261816275594433No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAATTATTATTTAAATTGGTATCAGCAGAAA17490.1710533522153805No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17190.1681193324518234No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16220.15863266854965535No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15910.15560084812731298No Hit
ACATATTACTGTCAACACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15880.15530744615095726No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15590.15247122704618538No Hit
CAGTAATATGTTGCAATATCCTCAGGCTGCAGACTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTCCCA14960.14630978554271543No Hit
ATCATAGTGTTGACAGTAATATGTTGCAATATCCTCAGGCTGCAGACTGC14870.14542957961364827No Hit
CACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14870.14542957961364827No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14520.14200655655616498No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13750.13447590582970168No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13690.13388910187699024No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13540.13242209199521168No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13480.13183528804250025No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13460.1316396867249298No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13450.13154188606614453No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13440.1314440854073593No Hit
GTAATATGTTGCAATATCCTCAGGCTGCAGACTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTCCCA13440.1314440854073593No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13260.12968367354922503No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC12960.1267496537856679No Hit
ATCTACGATGCATCGAATTTGGAGACAGGAGTCCCATCAAGGTTCAGTGG12960.1267496537856679No Hit
GTCTGCAGCCTGAGGATATTGCAACATATTACTGTCAACACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGA12680.12401123533968125No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12610.12332663072818457No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12290.12019700964705697No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12070.11804539515378176No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11950.1168717872483589No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC11940.11677398658957368No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11740.11481797341386892No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT11480.11227515628545275No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11440.11188395365031178No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11440.11188395365031178No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11390.1113949503563856No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11370.11119934903881512No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11290.11041694376853323No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACGT10850.10611371478198278No Hit
GCAGAAACCAGGGAAAGCCCCTAACCTCGTGATCTACGATGCATCGAATTTGGAGACAGGAGTCCCATCAAGGTT10760.10523350885291564No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10500.10269069172449946No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10440.10210388777178803No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10430.10200608711300278No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10390.10161488447786185No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10310.10083247920757994No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10300.10073467854879471No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10290.10063687789000947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)