FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022488
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38210.3736963172183928No Hit
CTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32790.32068836015679403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31430.3073874705620017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30190.2952601888726323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.2904679565921556No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29440.28792513946373943No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23700.23178756132101308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22880.22376790730062357No Hit
CTATCATAGTGTTGACAGTAATATGTTGCAATATCCTCAGGCTGCAGACTGCTGATGGTGAAAGTAAAATCTGTC21750.21271643285789174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21670.21193402758760985No Hit
ATATTACTGTCAACACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGA21590.21115162231732792No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG21480.21007581507069029No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20740.20283856632058272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20070.19628592218197183No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19490.19061348397242805No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATTAATTATTATTTAAATTGGTATCAGCAGAAA18750.18337623522232047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18610.18200702599932714No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17910.1751609798843605No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17900.17506317922557527No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17710.17320496670865576No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16890.16518531268826625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16790.1642073061004139No Hit
GCCCCTAACCTCGTGATCTACGATGCATCGAATTTGGAGACAGGAGTCCCATCAAGGTTCAGTGGGAGTGGAT16130.1577524626205882No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16060.15706785800909154No Hit
ATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA15710.15364483495160822No Hit
ACATATTACTGTCAACACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15180.14846140003599062No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15150.14816799805963493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14670.14347356643794354No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14600.14278896182644685No Hit
CACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT14300.13985494206288973No Hit
CAGTAATATGTTGCAATATCCTCAGGCTGCAGACTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTCCCA14150.13838793218111117No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14070.13760552691082928No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13530.13232429133642645No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13470.13173748738371502No Hit
ATCATAGTGTTGACAGTAATATGTTGCAATATCCTCAGGCTGCAGACTGC13380.13085728145464787No Hit
GTAATATGTTGCAATATCCTCAGGCTGCAGACTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTCCCA13360.1306616801370774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12890.12606504917417125No Hit
GTCTGCAGCCTGAGGATATTGCAACATATTACTGTCAACACTATGATAGTCTCCCTCTCACTTTCGGCGGAGGGA12560.1228376274342584No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12550.12273982677547315No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12190.11921900305920462No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12060.11794759449499652No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11790.1153069767077951No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11440.11188395365031178No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11400.11149275101517084No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11360.11110154838002988No Hit
ATCTACGATGCATCGAATTTGGAGACAGGAGTCCCATCAAGGTTCAGTGG11350.11100374772124466No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11320.11071034574488894No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11190.10943893718068085No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11100.10855873125161372No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10870.10630931609955324No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10860.10621151544076803No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC10680.10445110358263372No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC10510.1027884923832847No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10500.10269069172449946No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10430.10200608711300278No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10320.10093027986636519No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAG10320.10093027986636519No Hit
GATTTTACTTTCACCATCAGCAGTCTGCAGCCTGAGGATATTGCAACATATTACTGTCAACACTATGATAGTCTC10240.10014787459608326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG252.8979284E-453.861494
GTCGGAT200.006983492552.43953347
ATCGCAC200.00808832350.52727517
CTAACGC352.6284308E-548.0882643
CCTAACG405.7760415E-542.085462
GTATCAA35250.039.3648831
CTATACC802.0863808E-937.8713574
GTGGTAT3650.035.9867481
TATCAAC38600.035.4128462
GCGACGA400.002704320234.21405437
ATCAACG40150.034.206743
GATCGTC400.00284559833.86053529
TCAACGC40850.033.787044
AACGCAG41150.033.4621856
CAACGCA41450.033.2995955
CGCAGAG42900.031.9417888
ACGCAGA43600.031.5061827
AGAGTAC43850.031.09469211
CAGAGTA44500.030.79331810
CGACCCA450.004614142730.6778841
Produced by FastQC (version 0.11.5)