FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114964
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA35740.32054846613881705No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35180.31552588245001634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29730.2666453804786522No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26660.23911085918469116No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26290.23579236639030499No Hit
GGCCAAGACTGTGAGGCCAGAATGAACCTTGCATGCAATAATAAACCCCA24920.2235049741516318No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23740.21292167280737317No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23290.20888566805744402No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23040.20664344319637223No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA22610.20278681643532886No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21310.19112724715775578No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA20350.18251710369124027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18780.16843593156370967No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16430.14735901786963526No Hit
CCCTTGGACAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAAAGACTCGTCCACAGTGATGGAAACACGTACTTGA16380.1469105728974209No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16360.14673119490853515No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG16120.14457865904190628No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15500.13901794138644835No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15070.13516131462540495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14810.13282940076989033No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14270.12798619506997536No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13920.12484708026447491No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13810.12386050132560335No Hit
GTCTTCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAAAGACTCGTCCA13670.12260485540340316No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13590.1218873434478602No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12820.11498129087575923No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12820.11498129087575923No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12660.1135462669646733No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12610.11309782199245895No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12210.10951026221474415No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12130.10879275025920118No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11930.10699897037034378No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11310.10143825271488588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT651.4481053E-553.181470
GGTATCA13950.046.4518131
CTAACGC752.1827873E-1144.969913
TGACCGA350.001028696141.65500662
GTGGTAT3100.039.1937181
GTATCAA35700.038.287981
CATCTGA17950.037.7454670
CCTAACG1004.8567017E-1033.7319762
CGACGCA450.003153981633.1570266
TATCAAC42000.032.286322
TGGTATC3650.031.4215662
ATCAACG42950.031.3323553
TCAACGC42950.031.17674
CAACGCA43550.030.747175
AACGCAG44400.030.3104746
CGCAGAG46950.028.5923798
CCGACGC655.7623285E-428.45219265
CAGTTCG10250.028.32882170
TGTTAGA11750.028.24271670
GCAGAGT48100.028.1191449
Produced by FastQC (version 0.11.5)