FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856354
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT75240.4053106250208742No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC60600.3264463566755048No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA58790.3166960612038437No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55500.29897314844043754No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC53790.28976154332632675No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC52870.2848055920368637No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG51020.2748398204221824No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTATATGAGCTGCAGTAATAAT49110.2645508345929709No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC47950.258302026445387No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT46630.25119131372572256No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT42320.227973759315303No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGC41620.22420292681245066No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG40900.22032435623808821No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT38520.20750352572839015No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT37370.20130858661656126No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG36900.19877674193607467No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36470.19646037339860825No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34620.186494601783927No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTATATGAGCTG33800.1820773408520142No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG33380.1798148413503028No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGC32540.17528984234687997No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG29910.16112228594330608No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29520.15902139354885975No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC29370.15821335801253425No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTG28950.15595085851082283No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG28230.15207228793646038No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27720.14932496711295368No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACCGTGTCTCCTGGTACCAGCAGCC26720.14393806353745028No Hit
TTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAAC25830.1391437193552523No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA25360.13661187467476568No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC24680.13294878024342338No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGTTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24520.13208687567134286No Hit
CCACCAAACCCTCCAAACAGAACAACAACAAGTACGCGGCCAGCAGCTAC24410.13149431627803748No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG23900.12874699545453078No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23710.1277234837751851No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23380.12594580559526902No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC23090.12438360355837302No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG22670.12212110405666161No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC22310.12018181876948038No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22180.11948152130466494No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGT21320.11484878422973205No Hit
GCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC21090.11360979640736626No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20860.11237080858500048No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20850.11231693954924546No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC20580.11086247558385953No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC20310.10940801161847365No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20150.10854610704639309No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT19840.10687616693798704No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGACCCAGAGAAG19520.10515235779382597No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG19480.10493688165080582No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19440.10472140550778569No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19180.10332081057815481No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19180.10332081057815481No Hit
GAACAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG19050.10262051311333938No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18870.10165087046974876No Hit
GTGCCTGGGGGCTGCTGGTACCAGGAGACACGGTTATAATTACCAACGTC18680.10062735879040313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT551.5256956E-743.70335439
TAAGGCG3900.042.55336429
GCGAATC3150.042.14365833
GGCGAAT3650.040.97337732
GACTAAG4350.040.41176626
GGTATCA22850.040.2211041
AATCGCG857.2759576E-1140.21632836
ACGAGAC4150.039.88432722
CGAATCT2250.039.38968734
CGAATCG1150.038.53338634
CGAGACT4300.038.49612423
CCCACGA4300.038.48575619
AGACTAA4400.037.63337725
GTATCAA59300.036.979521
CGTATGC1500.036.7776541
GAGACTA4550.036.38095524
ACTAAGG4650.036.36131727
GCGGCAT1753.265086E-936.17340570
TCTCCGA4850.035.51218811
AAGGCGA4800.034.6082630
Produced by FastQC (version 0.11.5)