FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856354
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT68900.37115765635218284No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC62410.3361966521471659No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC54970.29611808954542074No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA54370.2928859474001187No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC53860.29013862657661205No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTATATGAGCTGCAGTAATAAT50950.2744627371718972No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG50210.27047642852602466No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48760.2626654183415448No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC45470.24494250557813863No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT44900.24187197054010173No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG39960.21526066687711504No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT38410.20691096633508477No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37850.2038943003328029No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT37560.2023320982959069No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35320.19026543428677936No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT35050.18881097032139343No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGC34450.18557882817609142No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34410.18536335203307128No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG34150.1839627571034404No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG32950.17749847281283634No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTGCTGCTTGTATATGAGCTG32710.17620561595471554No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG32010.17243478345186317No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGC31010.1670478798763598No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTG30080.16203805955114164No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29800.1605297265500007No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG27540.14835532446936306No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC27020.1455541346101013No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26490.14269907571508453No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC26250.14140621885696372No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC25340.13650413660325564No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG24860.133918422887014No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGTTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA24630.13267943506464824No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23990.12923181677632609No Hit
TTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCACAGCCCCCAAAC23870.12858538834726566No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACCGTGTCTCCTGGTACCAGCAGCC23650.12740026956065492No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC23220.12508390102318848No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23170.1248145558444133No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA22740.12249818730694685No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC21910.11802705733927904No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21560.11614164108785287No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21400.11527973651577232No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21100.11366366544312131No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21070.1135020583358562No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20980.1130172370140609No Hit
CCACCAAACCCTCCAAACAGAACAACAACAAGTACGCGGCCAGCAGCTAC20700.11150890401291995No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT20590.11091634461961458No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGT20580.11086247558385953No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19900.10719938115251725No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGTT19820.10676842886647697No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19330.10412884611448031No Hit
GCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC19240.103644024792685No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19130.10305146539937964No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19030.1025127750418293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5650.041.2197531
GGTATCA23000.038.888641
GTATCAA54650.035.5125961
TATCAAC59500.032.3202972
AATGGTA9400.032.05860570
ATCAACG60900.031.740083
TCAACGC61100.031.4722444
CAACGCA61600.031.1628635
AACGCAG63000.030.7924886
ACGCAGA66600.029.0267147
ATACACG704.487991E-528.9166014
CGCAGAG66650.028.9036968
TACGTAT1504.7211433E-528.70008570
AGAGTAC67800.028.46243711
CAGAGTA69150.028.10268410
GCAGAGT69600.027.775569
TAGTCGT550.00788139427.49479567
TGGTATC9050.026.095582
ATGGCAA8000.026.00945370
GTGGTGA12250.025.1857970
Produced by FastQC (version 0.11.5)