FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189041
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42740.35944933774361015No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA38020.3197534820077693No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG31870.2680311276061969No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31860.26794702621692607No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31120.26172352341088323No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30950.26029379979327877No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTGCTGCTTGTATATGAGCTGCAGTAATAAT28370.23859564136139966No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACACTTATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGT28130.23657720801889925No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27670.23270854411244019No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25790.21689748292952052No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25760.216645178761708No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT24280.20419817314962224No Hit
ACTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCGCAGCCCCCAAACTCATGATTTTTGACGTCAGTAAT23370.19654494672597497No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAACACTTATGTCTTCG22310.18763019946326495No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTGCTGC21990.18493895500659774No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT21110.17753803275076302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20840.17526729524045007No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20550.17282835495159543No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG19910.1674458660382611No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTGCTGCTTGTATATGAGCTG19590.1647546215815939No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCGCAGC19460.1636613035210729No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19330.16256798546055182No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTG18460.1552511645939879No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACCGTGTCTCCTGGTACCAGCAGCC17560.14768203955961148No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17540.1475138367810698No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17540.1475138367810698No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16920.14229955064627714No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG16840.14162673953211033No Hit
GACATAAGTGTTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGT16640.13994471174669335No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC16500.13876729229690146No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16360.13758987284710958No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16240.13658065617585938No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15950.13414171588700474No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15560.13086176170544161No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTA15090.1269089964097117No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGT13720.11538710607960533No Hit
GTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG13240.11135023939460456No Hit
CACTTATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT13210.11109793522679201No Hit
GTAATACAAGGCCGAGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA13150.11059332689116691No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12950.10891129910574993No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12670.10655646020616616No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12450.10470622964220747No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12450.10470622964220747No Hit
GCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC12250.10302420185679048No Hit
GAATATCTCCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGT12180.10243549213189453No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12100.10176268101772773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)