FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189041
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT37650.3166417306047479No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37590.3161371222691228No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35630.2996532499720363No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG30530.2567615414439031No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC29770.25036983585931855No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28130.23657720801889925No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTGCTGCTTGTATATGAGCTGCAGTAATAAT27510.23136292188410662No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACACTTATGTCTTCGCAACTGGGACCAAGGTCACCGTCCTAGGT26960.2267373454742099No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26830.22564402741368883No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26600.2237096954604593No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT24390.20512328843160157No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22730.1911624578126406No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22600.19006913975211956No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21580.18149079804649293No Hit
ACTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCGCAGCCCCCAAACTCATGATTTTTGACGTCAGTAAT21020.17678112024732537No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAACACTTATGTCTTCG20860.17543549801899178No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTGCTGCTTGTATATGAGCTG20820.17509909246190838No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20660.1737534702335748No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACCGTGTCTCCTGGTACCAGCAGCCCCCAGGCGCAGC20030.1684550827095113No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20010.16828687993096958No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTG19390.16307259379617692No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19290.16223157990346843No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTGCGAAGACATAAGTGTTGCTGC19120.160801856285864No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC18490.15550346876180046No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17920.15070968957336206No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG17760.14936406734502847No Hit
GACATAAGTGTTGCTGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGT17150.14423388259950667No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG16870.14187904369992288No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16810.14137443536429778No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16240.13658065617585938No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16220.13641245339731767No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTTATAACCGTGTCTCCTGGTACCAGCAGCC16190.13616014922950512No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15850.13330070199429625No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15550.13077766031617077No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC15340.12901153114148295No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC15260.12833872002731614No Hit
GCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTA14170.11917166859679354No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCCGTGTCCGGGT13260.11151844217314627No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13070.10992051577700013No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC13050.10975231299845843No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13030.10958411021991672No Hit
GCCCTGACTCAGCCTCCCTCCGTGTCCGGGTCTCCTGGACAGTCAGTCAC13030.10958411021991672No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12570.10571544631345765No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT12470.10487443242074918No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12430.10453802686366577No Hit
GTTATAAGTACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG12420.10445392547439492No Hit
GAATATCTCCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGT12130.10201498518554028No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11930.1003329574001233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.4627676E-678.2651370
GGTATCA11500.045.0286251
GTACGTA557.486768E-540.27336569
CGTGCGG350.001436387838.92142510
GTATCAA29150.035.6454621
GTGGTAT3350.033.5592351
TGGTATC3400.033.0545922
TATCAAC32600.031.2356362
ATCAACG32400.031.2129753
CTAATGG1153.742729E-430.62548470
TCAACGC32950.030.5924874
CTATATC604.259934E-429.95940264
CAACGCA33850.029.880965
AACGCAG34750.029.4010756
CCGAAAC954.3024866E-628.5166568
TGGTACG655.882322E-428.35237567
TTGCGGT500.00693754728.22210156
AATGGTA5650.027.42742770
ACGCAGA37300.027.3910837
CGCAGAG37400.027.1357238
Produced by FastQC (version 0.11.5)