FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508277
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATCCACTGTACAAAGTGGGGTCTCATTGAGGTTCAGCGGCAGTGGA70280.46596215416664183No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65830.43645828982342105No Hit
GTACAAAGTGGGGTCTCATTGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAATACCCTG56650.37559413821201276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54340.3602786490810375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44980.2982210827321507No Hit
GTGGATATAGAATGCATTATGGACTTAGGGGCTTCCCCTGGTTTTTTCTG41870.2776015281012705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41420.274617991257574No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38090.25253981861422004No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34900.23138985743334944No Hit
ACTTTGTACAGTGGATATAGAATGCATTATGGACTTAGGGGCTTCCCCTG34280.2272792066709232No Hit
CAATCAATACCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAA33610.22283705181475288No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTACCACTTTTTTAGCCTGGTATCAGAAAAAA33310.22084802725228855No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC33260.22051652315854448No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32910.2181959945023361No Hit
ACAGTGGACATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT32490.21541136011488607No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31280.20738896104627993No Hit
ACTGAATACTTATCCTTTCACTTTCGGCCCTGGGACGACAGTGGACATCAAGCGAACTGTGGCTGCACCAT29980.19876985460893457No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29850.19790794396520006No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGACGTTACCA29660.19664822840897264No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29450.19525591121524757No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG29410.19499070794025236No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29410.19499070794025236No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28940.19187456945905823No Hit
ATCAATACCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAACTGAATACTTATCCTTTCACTTTCGGC28650.18995184571534274No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28600.1896203416215987No Hit
GTGCAGCCACAGTTCGCTTGATGTCCACTGTCGTCCCAGGGCCGAAAGTG27730.18385217039045215No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT26440.17529936477185556No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26310.17443745412812103No Hit
GTATTCAGTTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGGTATTGATTGTGAGAGTGAATTCTGTC25630.1699289984532019No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25560.16946489272196022No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25420.16853668125947688No Hit
GTCCACTGTCGTCCCAGGGCCGAAAGTGAAAGGATAAGTATTCAGTTGTT25270.16754216897824473No Hit
GTATTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCT24770.16422712804080417No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT24420.1619065993845958No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24340.1613761928346053No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24050.1594534690908898No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23290.1544146068659802No Hit
GTCAGGACGTTACCACTTTTTTAGCCTGGTATCAGAAAAAACCAGGGGAA22640.15010505364730747No Hit
GAATTCACTCTCACAATCAATACCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAACTGAATACTTAT22530.1493757446410706No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22250.14751932171610388No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21810.1446020856911562No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20760.13764049972253106No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20340.134855865335081No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20310.1346569628788346No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19310.1280268810039535No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19270.12776167772895827No Hit
GTCCATAATGCATTCTATATCCACTGTACAAAGTGGGGTCTCATTGAGGT19090.12656826299147966No Hit
ACTTATTACTGTCAACAACTGAATACTTATCCTTTCACTTTCGGCCCTGGGACGACAGTGGACATCAAGCGAA18890.12524224661650346No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC18530.12285541714154627No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18200.12066749012283552No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATGTCCACTGTCGTCC17900.11867846556037122No Hit
GGGTATTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAAC17690.11728614836664618No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17410.11542972544167948No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17400.11536342462293067No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG16860.1117831804104949No Hit
CATTGAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAATACCCTGCAGCCTGAAGATTTTG16850.11171687959174609No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16780.1112527738605044No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16510.10946265175428652No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16420.10886594438554723No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16340.10833553783555674No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT16260.10780513128556624No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15880.10528570017311142No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15480.10263366742315902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTT408.565803E-751.48443238
TCGCGTT200.007510677451.48443238
GCACGAC250.00963088448.3445468
CCTAACG452.2410568E-644.777712
TTGCGCG504.6186633E-640.30795718
ACGAGAC5150.039.80465322
TACGTAT1758.913048E-1139.45619270
GCATCGG1850.038.70153435
CGAGACT5350.038.32425723
ATCTCCG5450.038.20103510
CCCACGA5400.037.9454819
CTTATAC6000.034.7096331
GAGACTC6100.034.17008224
CGTACCC400.002963065233.5832823
CGAGCCC6100.033.5810615
ACTCGCA450.00300562133.48544766
TATACAC6350.033.3188443
TACACAT6250.033.3146175
GTATCAA38150.033.2818031
CCGAGCC6550.032.29826714
Produced by FastQC (version 0.11.5)