FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379115
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAGAGACG43770.3173774485811553No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42820.31048897300080125No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31230.2264495709204816No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAAGATCACTATAATCATCCCACAG29400.21318019164464166No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27950.20266620260094337No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25710.18642390228516115No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAAGATCACTA24280.1760549337799966No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23930.17351707435565564No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20900.1515464627677895No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20510.148718562266381No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20310.14726835688104328No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19940.14458547691816853No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19770.1433528023406315No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19670.14262769964796265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19190.13914720672315217No Hit
GGATTACCTGTGGGGATGACAATTTTGGAACTAAAAGTGTGCACTGGTACCAGCAGAAGACAGGCCAGGCCC19050.1381320629534158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18660.13530416245200727No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18370.1332013646432676No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18020.13066350521892664No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17080.12384753990783944No Hit
CTATTACTGTCAGCTGTGGGATGATTATAGTGATCTTGTGGTCTTCGGCG17070.12377502963857255No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17000.12326745775370436No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16180.11732161567381981No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15790.11449371517241128No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATTATGATAGAGACCGGCCCTCAGG15240.11050565036273262No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14940.10833034228472607No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14690.10651758555305395No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14590.10579248286038509No Hit
CCCCAGGAGAGACGGCCAGGATTACCTGTGGGGATGACAATTTTGGAACT14470.10492235962918249No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14330.1039072158594461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.046.7001881
CTAGCGC2400.046.55775528
TAGCGCT2650.043.47643729
CGTATGC1350.040.91271641
GTATCAA53150.038.7794271
ACTAGCG2900.038.50816327
GACTAGC3050.036.59305226
CGCTCAT2700.036.5156632
GCGCTCA3100.036.13796231
CGAGACT3250.035.34332323
GCGTAGA1001.9252008E-434.30023670
ACGAGAC3350.034.288322
TATCAAC60700.033.784062
ATCTCCG3600.033.77793510
TCGTATG1550.033.3472440
CCCACGA3700.032.86978519
ATCAACG62550.032.783663
TCAACGC63000.032.495874
CGAGCCC3850.032.4630915
CACGAGA3700.031.95789521
Produced by FastQC (version 0.11.5)